Technical BioImaging

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Technical BioImaging
TIF045 5p 24th October-9th December

• To see is to understand...

http//www.fy.chalmers.se/atom/research/cars/cours
es
Contact Annika Enejder, enejder_at_fy.chalmers.se
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Technical BioImaging
Aims

• To understand how a 3-dimensionell, highly
  resolved image is created in a modern light
  microscope
• The physics behind light-matter interaction in a
  microscopic probe volume
• How cell structures and even biomolecules can be
  imaged and manipulated in a microscope
• Give a flavor of all the novel microscopy
  techniques currently emerging within the exciting
  inter-disciplinary field of physics and molecular
  cell biology

Contact Annika Enejder, enejder_at_fy.chalmers.se
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Technical BioImaging High-lights from the
program

• The Nikon Microscopy Workshop at Chalmers
  University
• 26-27th October
• From the fundamentals on the anatomy of a modern
  microscope to hands-on training in how to use a
  microscope collect images of biological samples

Contact Annika Enejder, enejder_at_fy.chalmers.se
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Project conducted on the research microscopes at
The Centre for BioPhysical Imaging14-18th
November
Technical BioImaging High-lights from the
program

• Confocal microscopy of GFP labeled proteins
• 2-photon fluorescence microscopy
• Fluorescence Lifetime Imaging FLIM
• CARS - Coherent Anti-Stokes Raman Scattering
  microscopy
• Vibrational (Raman) microscopy
• Surface Plasmon Resonance and Nanoparticle
  sensors
• Optical tweezers and manipulation

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Technical BioImaging High-lights from the
program

• Lecture series by Prof James B Pawley
• University of Wisconsin, Madison, USA
• 21-23th November

The editor of the course literature Handbook of
Biological Confocal Microscopy
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Technical BioImaging Course Contents

• The light microscope has traditionally been our
  most important instrument to catch a glimpse of
  the fascinating micro-cosmos of biological cells.
  The fundamental optics of the microscope and its
  function to image with an ever increasing
  magnification and resolution has been
  well-known for several hundred years. However,
  the information provided by the conventional
  snapshots of transmitted light is no longer
  enough within the modern biosciences in order to
  gain full understanding of the complex processes
  life comprises. The requirements on resolution
  and contrast in order to visualize the
  invisible have the past decade drastically been
  changed we now want to be able to monitor
  biochemical processes in living cells on
  molecular level and with high temporal resolution
  rather than merely visualizing cellular
  structures. This has motivated physicists in
  close collaboration with biologists to develop a
  range of sophisticated microscopy methods, where
  the conventional light source is replaced by
  technically advanced laser systems.
  Micro-spectroscopic analysis and imaging of
  biomolecules and molecular markers in the sample
  is done by inducing fluorescence and vibrational
  processes. Living cells are captured in the probe
  volume and manipulated with laser tweezers. The
  light emitted from the sample, sometimes only
  single photons, is wavelength-selectively
  filtered and projected onto a sensitive detector.
  An image is formed and finally analyzed by means
  of powerful image analysis software. These
  advanced laser-based imaging systems set high and
  new requirements on the user of modern
  microscopes, one of which is good knowledge in
  physics. Here we intend to bring up concepts from
  a wide range of fundamental physics courses and
  use them to understand how a 3-dimensionell
  picture is created in a modern light microscope,
  how cell structures and biomolecules selectively
  can be imaged and manipulated in fluorescence and
  spectroscopically based microscopes, and how
  important image analysis and sample preparation
  is for the final results.

Contact Annika Enejder, enejder_at_fy.chalmers.se
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Technical BioImaging
TIF045 5p
For further information, contact Annika Enejder
enejder_at_fy.chalmers.se
http//www.fy.chalmers.se/atom/research/cars/cours
es