Welcome to the continuation of Biol 213 Genetics!

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Welcome to the continuation of Biol 213
Genetics!
Genetics News
Whats in the mail?

• EditBase - DNA processing program

• Mystery sequence

Problem Set 3 now available
Lab 2 - long
Feedback welcome
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Upcoming events
Monday, Sept 18 Problem day (Problem Set 3)
Friday, Sept 22 Problem day (Problem Set 4)
Monday, Sept 25 First exam (700 PM)
Monday, Sept 25 in class.?
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Todays events
DNA Replication Integration of
leading/lagging strand synthesis
Inborn errors of metabolism How does
alkaptonuria work
Beadle Tatums experiment with Neurospora
How does alkaptonuria work
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DNA Replication
How can DNA polymerase go left to right and
right to left simultaneously?
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Relationship between genes and proteins
One gene - many protein
gene
RNA
Proteinprecursor
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Relationship between genes and proteins
One gene - many protein
Proteinprecursor
lactose
maltose
Lactose degrading enzyme
Maltose degrading enzyme
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Relationship between genes and proteins
Many genes - many protein
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Relationship between genes and proteins
Many genes - many protein
One gene - One protein
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Phenylalanine degradation
Dietary protein phenylalanine other
amino acids tyrosine p-hydroxypheny
lpyruvic acid homogentisic acid
maleylacetoacetic acid
Homogentisicacid oxidase
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Phenylalanine degradation
Dietary protein phenylalanine other
amino acids tyrosine p-hydroxypheny
lpyruvic acid homogentisic acid
maleylacetoacetic acid
Black oxidation product
Homogentisic acid
Alkaptonuria
Homogentisicacid oxidase
Problems
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Phenylalanine degradation
Dietary protein phenylalanine other
amino acids tyrosine p-hydroxypheny
lpyruvic acid homogentisic acid
maleylacetoacetic acid
Phenylalaninehydroxylase
Phenylketones (neurotoxins)
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Life cycle of yeast
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How to get mutants in Neurospora
Haploid Neurospora
Diploid Neurospora
radiation
radiation
screen 1000s
Auxotroph?
No auxotrophs
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How to get mutants in Neurospora
self cross1000s
auxotroph?
Could you do this with humans?
back cross1000s
self cross
mixed mating types
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Analysis of Beadle Tatum Expt
1. One gene - many enzymes 2. Many genes - one or
more enzymes 3. One gene - one enzyme