Similar resolution as CLSM PowerPoint PPT Presentation

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Title: Similar resolution as CLSM


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Advantages of multi-photon excitation microscopy
  • Similar resolution as CLSM
  • No cytotoxicity by harmful UV
  • Reduced photobleaching (confined to focal plane)
  • Can image live cells and tissue for much longer
    time than CLSM
  • Images much deeper than CLSM due to absence of
    out of focus absorbtion excitation light.
  • no pinhole required, increased sensitivity.
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