RNAi mechanisms in C' elegans

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RNAi mechanisms in C. elegans
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Analysis of RNA-interference effects in
individual cells
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Effect of site of injection
Characteristics of the RNAi mechanism in C.
elegans -systemic -heritable when genes
expressed in the germline are targeted

• Methods
• injection of dsRNA
• feeding
• soaking

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Feeding vectors
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dcr-1 mutants are defective in RNAi
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Germ line development is abnormal in dcr-1 mutants
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Functional classes of STE, EMB, UNC and PEP genes
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Mechanism of RNA interference Isolation of
RNAi-defective mutants
GFP dsRNA
GFP dsRNA
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RNAi mechanisms
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LIN-29GFP
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The 21-nucleotide let-7 RNA
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Regulation of heterochronic genes by the lin-4
and let-7 RNAs
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let-7 regulation of heterochronic genes
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Predicted secondary structures of stem-loop
precursors of selected C. elegans miRNAs
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Expression of C. elegans miRNAs
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Co-suppression of mrt-2