The PHILIS Support Contract to the U'S' EPA National Decontamination Team

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The PHILIS Support Contract to the U.S.
EPANational Decontamination Team

• Site Visit for the Portable High-Throughput
  Integrated Laboratory Identification System
  (PHILIS)July 21st, 2009
• National Decontamination Team
• US EPA Special Teams

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So what is the PHILIS ?

• It is a national asset of the EPA for the
  analysis of CWAs/ITCs on site
• Collection of mobile analytical systems at 3
  locations across the US
• It is a full function lab on wheels performing
  EPA validated methods producing data of known
  QA/QC integrity
• It is not a disaster only asset

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PHILIS Mission

• Fill perceived EPAs gaps in the nations capacity
  to analyze for CWAs in environmental samples
• Provide on-site analysis for TICs and CWAs
• Reduce time to recovery for critical
  infrastructure
• Validate (through the ERLN) CWA analytical
  methods
• Dual Use Add to EPAs analytical assets
• Provide surge capacity for EPA Regional and
  Program laboratories in response to non-WMD
  events natural disasters, accidents, spills
• On-site analytical assets on EPA Superfund clean
  up sites
• Train chemists for EPAs Laboratory Support
  Response Corps

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A Little Background

• EPAs Chemical Surety Program
• PHILIS 1
• PHILIS 2

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PHILIS Goals

• Provides the EPA with mobile, on-site analytical
  capability for the analysis of CWAs in soil,
  surface, water and air matrices
• Augment EPAs capacity to analyze CWAs in an
  emergency response (surge capacity)
• Participate in validation of EPA analytical
  methods (SAM) for the CWAs
• Provide real-time, on-site data to EPAs response
  and remedial staff for the timely, cost-effective
  mitigation and recovery of site contaminated by
  CWAs (or TICs/TIMs) due to a terrorist attacks
• Provide analysis of TICs/TIMs in the event of a
  natural disaster or accidental spills or releases
  

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PHILIS 1 to PHILIS 2

• The best aspects of the three PHILIS 1 systems
  were used to construct the new PHILIS 2 prototype
  (PHILBERT son of PHILIS)
• GC/MS w/FPD
• Pressurized solvent extraction sample prep (PSE)
• LC/MS-MS added
• LIMS
• The remaining PHILIS 1 assets were used as a
  starting point to construct new PHILBERT systems
  (pending in NJ and CO)
• 10 GC/MS systems (NJ)
• 3 TOF GC/MS systems (CO)
• SPE sample prep (CO)
• 1 LC/MS-MS added (NJ)
• 2 - P T systems added (NJ)

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Upgrade Existing PHILIS -1 assets to PHILIS-2
capabilities

• Automated sample prep
• Ultra-dilute Agent Security Room
• GC/MS system
• LC/MS-MS system
• Wireless, encrypted LIMS

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PHILIS 1 - NJ

• PHILIS 1 units arrived in Edison, NJ and Erlanger
  KY in July 2007. Currently running under
  temporary shore power and shelter
• Currently capable of only analyzing TICs (method
  8260/8270)
• Edison unit includes 10 GC/MS instruments and a
  sample prep trailer. Two P T, LC/MS-MS
  recently (October 2008) added.
• A Sample Log-in and Area (SLA) trailer needed
• NDT will work with ERT and Region 2 emergency
  response program and laboratory on initial set up
  of lab.

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NJ PHILIS
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PHILIS Status - NJ

• Have Modified PHILIS 1 Systems
• 2 24 bed lab trucks (APL01, APL02)
• 1 24 bed 5th wheel trailer w/Ford 550 for
  sample prep (SPA)
• 1 12 bed refrigerated box truck
• 10 GC/MS systems
• 2 PT systems for VOCs
• 1 LC/MS-MS system (not in PHILIS, in Region 2
  fixed laboratory)
• Need Finalize Permanent shore power and shelter
  solution
• Warehouse space construction completed, HS
  issues linger
• Support offices, storage, meeting/training rooms
• Link in with Region 2 and EPA Lab Response Corps
• SLA trailer w/UDA CWA standards secure room
• LIMS upgrade underway

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PHILIS 1 CO

• Denver PHILIS unit includes sample prep trailer
  and mobile laboratory bus with 3 time of flight
  (TOF) GC/MS systems.
• Warehouse construction is currently underway,
  with a late August completion date
• Automated sample prep equipment and a SLA trailer
  needed
• Units have potential to accelerate GC analysis
  time. Rapid GC/TOF-MS methods currently being
  developed and validated by NHSRC and NEIC

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CO PHILIS
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Time of Flight (TOF) GC/MSCO PHILIS

• Fast GC and TOF/MS

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PHILIS Status - CO

• Have Modified PHILIS 1 System
• 1 45 mobile lab RV (PAL)
• 1 53 sample prep trailer (SPA)
• 3 TOF GC/MS systems w/rapid GC
• 3 SPE systems for SVOCs
• Need - Permanent shore power and shelter solution
• Complete construction on warehouse, end of August
  2009
• Link in with NCERT/NEIC for TOF methodologies
• SLA trailer w/UDA CWA standards secure room
• VOCs sample prep system (PT systems)
• Upgrade of SVOCs sample prep system (PSE systems)
• LIMS upgrade

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PHILIS 2 (PHILBERT) - KY

• Flagship PHILIS Unit
• Mobile laboratory consortium of 3 vehicles
• Analytical Trailer with 6 GC/MS systems and one
  LC/MS/MS system
• Sample Preparation Trailer for prep. operations
  of soil, water, air, and wipe samples.
• Sample Log-In and Standards (ultra-dilute CWAs)
  Storage trailer
• Anticipated Capacity of 100 200 Toxic
  Industrial Chemical or Chemical Warfare Agent
  samples per 24 hr. period
• Will analyze CWAs and their breakdown products
  using SW 846 methodologies found in Standardized
  Analytical Methods (SAM).
• Delivered April 2008, warehouse renovations to
  current building underway for shore power and
  warehouse/office space.
• Proposed location for CWA training and
  certification for EPAs Laboratory Response Corps
• Prototype for EPAs future PHILIS systems

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PHILBERT KYSPA and PAL Trailers
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PHILBERT KYSLA Trailer (UD CWA Secure Room)
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PHILBERT - KYLC/MS-MS System
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PHILBERT Status - KY

• Have One Complete PHILIS 2 system in KY
• 1 53 lab trailer (PAL)
• 1 53 sample prep trailer (SPA)
• 1 - 24 bed 5th wheel trailer w/Ford 550 for
  sample log-in (SLA)
• 6 - GC/MS w/FPD
• 1 - LC/MS-MS
• 3 - PSE automated sample prep for SVOCs
• 2 - P T systems for VOCs
• Wireless LIMS
• Need Permanent shore power and shelter solution
• Support offices, storage, meeting/training rooms
• HVAC renovations as per HS requirements (if
  required)
• Link in with Region 5/NHSRC LC/MS-MS methodology
  RD

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PHILIS-2 Data Flow
Sample In/ Screening COC generated/LIMS
initiated Ultra-dilute CWA standards
Analysis of CWAs/SVOCs/VOCs 6 GC/MS
w/FPD 1-LC/MS-MS
Step 2-SLA Sample Log-in
Step 4-PAL Sample Analysis
LIMS connected
Automated Sample Prep Soil, water, wipes, air,
solids PSE/SPE/ P T
Sampling Info Scribe Format
Data Output Scribe Compatible
Step 3-SPA Sample Prep
Step 1- START Sample Collection
Step 5 Report Out Data to OSC/UC/EU
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PHILIS Challenges

• Method development and validation
• SAM methods
• LC/MS-MS methods
• TOF rapid methods
• CWA breakdown products
• Integration of PHILIS into ERLN
• Upgrades of PHILIS 1 units to PHILIS 2/PHILBERT
  capabilities
• On-site analysis of CWAs and breakdown products
• Dual Use at PHILIS systems (ie Superfund sites)

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Site Visit Health Safety

• Slip, trips and spill hazards
• Watch for extension cords, cables
• Equipment on the floor
• Lighting not uniform
• Stairs and decks
• High heels/shoes can get stuck in gratings

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Questions

• NDT Director Steve Hawthorn
• PHILIS Operations Manager Larry Kaelin, NDT
• CWA Methods and Validation Terry Smith, OEM
• Contradictions to any of the above Schatzi
  Fitz-James