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Introduction to Rapid Stain Identification

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Introduction to Rapid Stain Identification ... adsorb stain onto swab. extract swab. batting in PBS. RSID. Running Buffer. mix extract aliquot ... – PowerPoint PPT presentation

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Title: Introduction to Rapid Stain Identification


1
Introduction to Rapid Stain Identification
Immunochromatographic Strip Tests for the
Identification of Body Fluids for Forensic
Laboratories
RSID - Saliva
2
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RSID Rapid Stain IDentification
Brought to you by
Jennifer Old, Ph.D. Liz Graffy, MS Brett
Schweers, Ph.D. Karl Reich, Ph.D. Others . . .
. . . .
Current Tests presumptive cross-reaction
non-specific widely distributed
4
SALIVA
Format Lateral flow immunochromatographic
strip test
Antigen Human Salivary a-amylase
Antibodies 2 Mouse monoclonals, non-overlapping
epitopes
Labeling Colloidal Gold
Sensitivity lt 1 ml saliva
Cross-reaction None observed to date (see list)
Test Read-out Visual
Assay Time 10 minutes
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SALIVA
6
SALIVA
7
SALIVA
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SALIVA
Summary
SEMEN
First confirmatory Tests
No observed cross reaction with other body fluids
(see list at booth), domestic animals
(see list at booth), exotic species
(see list at booth), body fluid
mixtures (see list at booth)
Fast Results in 10 minutes
Sensitive Detects as little as 1 ml of body
fluid
Efficient Assay procedure integrated in DNA-STR
analysis
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SALIVA
Jennifer Old Karl Reich - Jack Keehma - Brett
Schweers P.W. Boonlayangoor
www.IFI-Test.com/rsid.html
SEMEN
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