Title: Crystal Structure of the Ribosome at 5.5
1Crystal Structure of the Ribosome at 5.5 Ã…
Resolution
- Marat M. Yusupov,1 Gulnara Zh. Yusupova,1
Albion Baucom,1 Kate Lieberman,1 Thomas N.
Earnest,2 J. H. D. Cate,3 Harry F. Noller1 - 1 Center for Molecular Biology of RNA, Sinsheimer
Laboratories, University of California at Santa
Cruz, Santa Cruz, CA 95064, USA. 2 Berkeley
Center for Structural Biology, Physical
Biosciences Division, Lawrence Berkeley National
Laboratory, Berkeley, CA 94720, USA. 3 Whitehead
Institute, Cambridge, MA 01242, USA.
2- Describe the three-dimensional structure of the
70S ribosome of the completed Thermus
thermophilus containing mRNA and tRNA bound to
the A and E site at 5.5 Ã… resulution and to the A
site at 7 Ã… resolution. - Overrall structure of the 70s ribosome.
- The densityof tRNA, bound to the P site of the
70S ribosome, at 5.5 Ã… resolution. - Views of the stucture of the T.thermophilus 70S
ribosome. - Secondary and tertiary structure of 16S, 23S,and
5S RNA. - Differences between the conformations of
70Sribosomes and isolated subunits. - Stuctures of the intersubunit bridges.
- tRNA-ribosome interactions.
- Implications for the mechanism of translation.
3Electron density of tRNAMetf bound to the P site
of the 70S ribosome, at 5.5Â Ã…Â resolution
4Subunits of prokaryotic ribosomes(70S)
5- A30s??????
- B???(30S???,50S?)
- C?50S?????
- D???
- E???(50S?)
- F50S???(interface,)
- G300S???(A??180?)
- ??23SRNA???5SRNA???16SRNA???50S????A???P
???E??
70S??????
6Secondary and tertiary structures of 16S, 23S,
and 5S rRNAs (A16SRNA B23S, 5SRNA
CThree-dimentional fold of 16s RNA D
CThree-dimentional fold of 23S and 5s RNA.)
7Conformational differences between rRNAs in 70S
ribosomes and 30S and 50S subunits
8Intersubunit bridges
9Intersubunit bridges(continued)
10- tRNA-ribosome interactions
11Two views of the A-tRNA anticodon stem loop bound
to its codon in the 30S subunit A site
12- Interaction of E-tRNA with the ribosome.
- Secondary structures of 16S and 23S rRNA,
showing molecular contacts with A-tRNA (gold),
P-tRNA (orange), and E-tRNA (red)
13THANK YOU!BYE BYE!2002.09