Crystal Structure of the Ribosome at 5.5 - PowerPoint PPT Presentation

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Crystal Structure of the Ribosome at 5.5

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Yusupova,1* Albion Baucom,1 Kate Lieberman,1 Thomas N. Earnest,2 J. H. D. Cate,3 ... 2 Berkeley Center for Structural Biology, Physical Biosciences Division, ... – PowerPoint PPT presentation

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Title: Crystal Structure of the Ribosome at 5.5


1
Crystal Structure of the Ribosome at 5.5 Ã…
Resolution
  • Marat M. Yusupov,1 Gulnara Zh. Yusupova,1
    Albion Baucom,1 Kate Lieberman,1 Thomas N.
    Earnest,2 J. H. D. Cate,3 Harry F. Noller1
  • 1 Center for Molecular Biology of RNA, Sinsheimer
    Laboratories, University of California at Santa
    Cruz, Santa Cruz, CA 95064, USA. 2 Berkeley
    Center for Structural Biology, Physical
    Biosciences Division, Lawrence Berkeley National
    Laboratory, Berkeley, CA 94720, USA. 3 Whitehead
    Institute, Cambridge, MA 01242, USA.

2
  • Describe the three-dimensional structure of the
    70S ribosome of the completed Thermus
    thermophilus containing mRNA and tRNA bound to
    the A and E site at 5.5 Ã… resulution and to the A
    site at 7 Ã… resolution.
  • Overrall structure of the 70s ribosome.
  • The densityof tRNA, bound to the P site of the
    70S ribosome, at 5.5 Ã… resolution.
  • Views of the stucture of the T.thermophilus 70S
    ribosome.
  • Secondary and tertiary structure of 16S, 23S,and
    5S RNA.
  • Differences between the conformations of
    70Sribosomes and isolated subunits.
  • Stuctures of the intersubunit bridges.
  • tRNA-ribosome interactions.
  • Implications for the mechanism of translation.

3
Electron density of tRNAMetf bound to the P site
of the 70S ribosome, at 5.5 Å resolution
4
Subunits of prokaryotic ribosomes(70S)
5
  • A30s??????
  • B???(30S???,50S?)
  • C?50S?????
  • D???
  • E???(50S?)
  • F50S???(interface,)
  • G300S???(A??180?)
  • ??23SRNA???5SRNA???16SRNA???50S????A???P
    ???E??

70S??????


6
Secondary and tertiary structures of 16S, 23S,
and 5S rRNAs (A16SRNA B23S, 5SRNA
CThree-dimentional fold of 16s RNA D
CThree-dimentional fold of 23S and 5s RNA.)
7
Conformational differences between rRNAs in 70S
ribosomes and 30S and 50S subunits
8
Intersubunit bridges
9
Intersubunit bridges(continued)
10
  • tRNA-ribosome interactions

11
Two views of the A-tRNA anticodon stem loop bound
to its codon in the 30S subunit A site
12
  1. Interaction of E-tRNA with the ribosome.
  2. Secondary structures of 16S and 23S rRNA,
    showing molecular contacts with A-tRNA (gold),
    P-tRNA (orange), and E-tRNA (red)

13
THANK YOU!BYE BYE!2002.09
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