Clorox

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Clorox,Peridex 3 commercial Denture Cleansers
as Denture Disinfecting AgentsIn Vitro
Comparison Study

• A.A.DAHLAN ,G. RAMAGE, C. HAVEMAN, S. REDDING,
  JL. LOEZ-RIBOT, L.BRADLY.UTHSCSA,TX.

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Objectives

• Determine the lowest concentration of Clorox
  (Sodium hypochlorite)and Peridex (Chlorhexidine)
  that eliminate the biofilm metabolic activity
  (BMA) of C.albicans.
• Determine the effectiveness of solutions of three
  commercial denture cleansers(Efferdent ,Polident
  for Dentures Polident for Partials) against
  BMA of C.albicans.

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Definitions

• Biofilm a matrix-enclosed population of cells
  adherent to surfaces, intersurfaces,and/or each
  other in a complex three-dimensional structure.
• Denture Induced Stomatitis (Chronic Atrophic
  Candidiasis) a very common oral fungal infection
  among denture wearers caused principally by
  Candida albicans as a result of diminished host
  resistance.
• XTT metabolic assay spectrophometric assay that
  measure the metabolic activity of the biofilms.

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Significance of the study

• Disinfect dentures contaminated with
  microorganisms (usually C.albicans).
• Long term goal is to develop a simple,
  inexpensive
• effective protocol to disinfect dental
  appliances with or without metal components that
  can be easily accomplished by patients in their
  home.
• The use of XTT metabolic assay.

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Methods Material

• Organisms 4 strains of C.albicans were isolated
  from denture stomatitis patients
  (3153A,SC5314,6455 6309)
• Biofilm formation 100µl of standardized cell
  suspension(1x10cell/ml) was pipetted into
  selected wells of microtiter plates and incubated
  for 48 hours _at_37C then medium was aspirated and
  non-adherent cells removed by thorough washing
  with
• PBS.
• Disinfectants
• . Clorox added in serially double diluted
  concentrations up to 1/512th incubated for 30
  min. _at_ room Temp.
• Peridex added at 3 conc.(100, 50 25)
  incub. 30 min _at_ room Temp
• Efferdent , Polident-D Polident-P tab.
  were dissolved in 200 ml of sterile water . 2
  trailsincub. 30 min incub. 18 hr.

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• ve and ve controls were included
• Biofilms were washed three times in sterile PBS

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• XTT Reduction Assay
• 100µl aliquot of XTT was added to each prewashed
  biofilm and to control wells to measure the
  background XTT levels then plates were incubated
  in the dark for 1 hr _at_37C. A colormetric change
  of XTT, a direct reflection of the metabolic
  activity of the biofilm, was then measured in a
  microtiter plate reader at 490nm.

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Results
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The Effect of Clorox on C.Albicans biofilm
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The Effect of Peridex on C.Albicans biofilm
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The Effect of Polident -D,Polident -P
Efferdent on C.Albicans biofilm30 min. Tx
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The Effect of Polident -D,Polident -P
Efferdent on C.Albicans biofilm18 hr Tx.
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Conclusion

• There was a 100 decrease in the biofilm
  metabolic activity of all tested strains exposed
  for 30 min. to Clorox in concentration of 132
  and stronger. There was also a 100 decrease in
  the biofilm metabolic actvitiy of all tested
  strains except SC5314 when treated with Peridex
  in concentration of 100 50 for 30 min or
  Polident for dentures for 18hr.