TODAY

1
TODAY

• Introduction Lab Rules and Safety
• Begin Environmental Isolate

2
Introduction

• Phantom Students
• Roll Call
• Your T.A.
• Where you can find me
• Your T.A.A.
• Website
• http//www4.nau.edu/biology/bio205/

3
Syllabus (Tentative)

• Required Materials (Need to be purchased)
• Grading/Point Structure
• Late Policy
• Conduct within the Lab
• Attendance Policy
• Academic Dishonesty (Plagiarism)
• Outline of Labs and Reading Assignments
• Due Dates

4
Orientation to the Lab

• Overview of Lab Manual
• Lab Rules
• Page 7 or vii in the lab manual
• Tour of the Lab
• Check for materials in lab drawers
• Disposal of items that will be used in the lab
• Safety Quiz

5
Assignments

• Mini Writing Assignments
• Mini 1, Due Next week
• Pre Labs
• Those that are throughout the semester
• Lab Reports and Format
• Rubrics
• Worksheets

6

• Take Safety Quiz

7
New in Microbiology

• Allergic to Peanuts??
• Fungus may help
• Baking peanuts in a harmless fungus could make
  them safe for people to eat.
• Cost would stay about the same.
• What next?
• Possibly breeding a hypoallergenic peanut! (Not
  in the near future.)
• http//www.newscientist.com/channel/health/dn7476-
  fungus-could-make-peanuts-less-allergenic.html.
  June 2005

8
Begin Environmental Isolate

• This is a semester long project
• -Labeling
• Must be on the bottom of the plate
• Initials, date, section, Control or A or B
• -Medium In microbiology, any liquid or solid
  preparation made specifically for the growth,
  storage, or transport of microorganisms or other
  types of cells.

9
Properties of the Medium

• Once set, the agar is stable at temperatures up
  to 65C or higher.
• Few organisms are able to degrade agar although
  it does dehydrate.
• Agar is a transparent gelatin-like substance
  that is formed when a mixture of agar and water
  is heated to gt100C and then cooled gelling
  occurs at 40-45C.

10
More Properties

• Growth media is prepared by adding nutrients,
  selective agents, etc. to agar usually before
  autoclaving.
• Agar is produced by red-brown algae and is widely
  used as a base for many kinds of solid and
  semi-solid microbiological medium.
• Rhodophyceae algae (red-brown algae) is a primary
  source of agar.

11
Trypticase Soy AgarMedium used today

• -Trypticase Soy Agar (TSA)
• a general purpose medium
• agar-based containing a pancreatic digest of
  casein (trypticase, tryptone), a papain digest of
  soybean meal (phytone), and NaCl (0.5 w/v).
• -Trypticase Soy Broth (TSB)
• is the same general purpose medium without agar
  base.

12
Procedure for Environmental Isolate

• Demonstration
• How to label a plate
• How to remove cotton swabs
• How to streak a plate
• Supplies
• 3 plates (check for unwanted growth)
• Test tube with 3 cotton swabs
• Sharpies

13
How to Streak Environment

• ABSOLUTELY NO
• HUMAN CONTACT SPOTS
• DOOR KNOBS
• CARS

Agar Plate
Name, section, date, Experiment, instructor
Lid of plate
Plate, agar side up
14
Environmental Isolate

• Plate 1 Control
• Control is a reference point in a controlled
  experiment in which a set of conditions does not
  vary
• Plate 2 and 3 will be labeled A and B, then
  record their sources.
• Go out and find your environmental isolate
• NO collections from Your own person, door knobs,
  restrooms, animals and their feces.

15
Environmental Isolate

• Your plates will incubate for one week
• Next week, you will begin to work in partners