Biosensor applications of nanochips

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Bio-sensor applications of nano-chips

• Martin Kristensen and Nina Skivesen
• iNANO and Department of Physics and Astronomy,
• University of Aarhus,
• Building 1520, Ny Munkegade, DK-8000 Århus C,
  Denmark
• Chip fabrication was performed at DTU.

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Characterization
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Use index change instead of stress
Application of a cantilever Courtesy H. P. Lang.
NCCR. Basel, Switzerland
Our activity
Microbalance
Surface stress
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Solvent shift in transmission
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Experimentally observed shifts
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Application as bio-sensor
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Analysis of results
From the measurements a change in cut-off
wavelength of 0.2nm corresponds to a ?nC 0.003.
Assuming a refractive index of the proteins of
1.47 this corresponds to a protein concentration
of 20mg/ml. However, the change in cutoff
wavelength of 0.2nm in the measurements is seen
for a concentration of only 10µg/ml. Thus, the
sensor shows a 2000 times higher sensitivity than
this simple estimate of the sensor
performance! A protein mono-layer deposited on
the surface combined with a localized evanescent
field gives high sensitivity and selectivity.
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Plans for specific cooperation with the partners

• Collaboration with DTU and IMEC well established.
• From the other partners we hope to get a few
  samples and perhaps packaging of a sensor.
• We can offer design and characterization
  immediately, and soon also immobilization of
  selective proteins.