Nacetyl glucosamine NAG 1 and niacinamide N 2 have each been shown to reduce facial hyperpigmentatio

1
P 235
Topical Formulation Containing N-Acetyl
Glucosamine and Niacinamide Reduces the
Appearance of Hyperpigmented Spots on Human
Facial Skin
A. B. Kimball (M.D., M.P.H.)1, D. L. Bissett
(Ph.D.)2 L. R. Robinson (Ph.D.)2, J. Li (M.S.,
M.A.)2, K. Miyamoto (Ph.D.)2, T. L. Grosick
(B.A.)2, P. J. Matts (Ph.D.)2, and C. A. Berge
(B.S.)2, Harvard Medical School1 and The Procter
Gamble Company2
INTRODUCTION
CONCLUSION
OBJECTIVE
RESULTS
Clinical results In computer image analysis, the
formulation regimen containing 2 NAG plus 4 N
significantly reduced the appearance of facial
hyperpigmented spots vs. the vehicle regimen
(Figure 1). Figure 1 color image spot area
fraction total hyperpigmented area X 100

total measurement area
Figure 2 NCS melanin spot area fraction
total NCS melanin area X 100
total
measurement area
N-acetyl glucosamine (NAG) 1 and niacinamide
(N) 2 have each been shown to reduce facial
hyperpigmentation in clinical evaluation. In
small base testing 1, a combination of the two
was observed to be more effective than
niacinamide alone. The current large base size
study of the combination was done to confirm
effectiveness of the combination.
A product regimen containing N-acetyl
glucosamine plus niacinamide clinically reduces
the appearance of facial hyperpigmented spots vs.
baseline and vs. the UV placebo
regimen. References 1. Bissett et al., AAD
Annual Meeting 2006, Poster P 236. 2. Greatens
A, Hakozaki T, Koshoffer A, Epstein H,
Schwemberger S, Babcock G, Bissett D, Takiwaki H,
Arase S, Wickett RR, Boissy RE, Effective
inhibition of melanosome transfer to
keratinocytes by lectins and niacinamide is
reversible, Exper. Dermatol., 14, 498-508
(2005). 3. Preece, S., Cotton, S.D. and
Claridge, E, Imaging the pigments of skin with a
technique which is invariant to changes in
surface geometry and intensity of illuminating
light, Medical Image Understanding and Analysis,
2003, D. Barber (Ed.), 145-148. 4. Matts et al.,
Poster 219, AAD meeting 2006.
To clinically evaluate the effect of topical
N-acetyl glucosamine in combination with
niacinamide on the appearance of facial
hyperpigmented spots.
improved
METHODS
Double-blind, vehicle-controlled, full-face,
clinical study. Subjects with facial
hyperpigmentation (n 200, ages 40-60). March
May in Cincinnati, Ohio Vehicle regimen (n
100) AM moisturizer (SPF15) PM
moisturizer. Treatment regimen (n 100)
AM moisturizer containing 2 NAG 4 N
(SPF15). PM moisturizer containing 2 NAG
4 N.
All subjects used the same facial moisturizer
product in 2-week wash-out period and same facial
cleanser for entire study 2-week wash-out
period then 8-week (2X daily) treatment
period. Facial images captured at end
of the 2-week wash-out (baseline) after 4,
6, and 8 weeks of treatment. Hyperpigmented
spot image analysis of blind-coded images
Computer full color polarized image
analysis (hyperpigmented spot area) NCS
(non-contact full-face SIAscopeTM) for
hyperpigmented spot area and for
pigmentation evenness 3,4. No adverse events.
Additionally, significant reduction in spot
appearance by the 2 NAG 4 N formulation vs.
vehicle was noted in NCS NCS melanin evenness
(Figure 3). Figure 3 Evenness mean variance of
pigmentation brightness in NCS melanin map
improved
Significant reduction in spot appearance by the
2 NAG 4 N formulation vs. vehicle was also
noted in NCS spot area fraction (Figure 2).
improved
This work was supported in its entirety by
Procter Gamble.