I. Basics

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I. Basics

• Electrophysiology is the study of cell electrical
  activity resulting from ion channel function.

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Electrolyte composition of the cell
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Fundamental laws

• Nernst's equation Er RT/F ln Co/Ci
• Driving force Em - Er
• Ohm's law V R x I

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A simplified view of cell-cell communication

• Ligand-gated channels code for input

• Voltage-gated channels code for output

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Baseline
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? Na leak
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GABAA IPSPs
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GABAB IPSP
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Baseline
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Subthreshold AMPA EPSP
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Threshold AMPA EPSP
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NMDA EPSP
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NMDA GABAA PSPs
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II. Methods
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Selective antagonism of GABA responses in the
cuneate nucleus of the anesthetized rat
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Configurations of the patch-clamp technique
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Configurations of the patch-clamp technique
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Patch-clamp dates
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The voltage-clamp technique
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III. From channels to behavior

• The thalamocortical augmented response (AR)

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Circuits involved in the AR
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Cellular mechanism of the AR
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Behavioral component of the AR