MD ANDERSON

1
HGU, Edinburgh September 28, 2006
2
Maureen Neitz
3
Roorda Williams, Nature 1999
4
3
4
R5
2
5
7
6
1
5
hn
Rh1

R1-6

6
Rh3
Rh5
R7
R8
Rh4
Rh6
7
UV
UV
rh4
rh3
R7
rh6
rh5
R8
Green
Blue
8
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9
Differentiation of photoreceptors
R8
R6
R1
10
spalt transforms outer into inner PRs
3
1
4
5
2
5
7
6
1
wt
11
Differentiation of photoreceptors
R8
R6
R1
12
prospero specifies R7, senseless R8
R7
R8
pros
sens
13
Refinement of photoreceptor fate
R8
otd
R6
R1
spineless
14
spineless
15
spineless is necessary for the rh4/rh6 fate
wt
Rh5 Rh6
Rh3 Rh4
R7
R8
16
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17
Refinement of photoreceptor fate
R8
otd
R6
R1
spineless
18
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19
Thomas Labhart
20
Navigation using polarized light
21
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22
Hth spineless
23
What controls hth?
Ventral
IroC-lacZ
Hth
Dorsal
Ventral
24
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25
R7
R8
26
What is the signal between R7 and R8?
ss
R7
Rh3
R8
27
ss
R7
Rh4
Rh3
X
?
R8
Rh5
28
ss
R7
Rh4
Rh3
R8
Rh5
All Rh5 Lats/warts
29
warts
Rh5 Rh6
wt
melted
30
GMRgtwarts
Rh5 Rh6
wt
GMRgtmelt
31
Rh6, warts
32
melted -/-
warts-/- melted-/-
Rh5 Rh6
33
rh4
rh3
melted
warts
melted
34
R7
Lats
Melt)
yorkie
R8 Differentiation
35
Signal from R7 to R8 Activin
Medea
Transcription
36
baboon Activin receptor affects R8 rhodopsins
Rh5 Rh6
GMRgtbaboDN
wt
GMRgtbabo
37
rh4
rh3
Babo
melted
warts
melted
38
Are Rhodopsins involved in the fate of
photoreceptors?
Rh5 Rh6
39
rh61 mutant co-expression of Rh5 Rh6-GFP
Rh5 Rh6gtGFP
40
Rescue of rh6 mutants by rh6gtRh6
Rh5 rh6gtRh6 GFP
41
General retina
Rh3
Rh4
R7
R7
Rh5
Rh6
R8
R8
Lamina
Medulla
42
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43
Cajal Sanchez, 1915
Fischbach Dittrich, 1989
44
Javier Morante (y Cajal!)
45
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46
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47
Logic of the color vision circuit
R8
R7
Interneurons
R7 vs. R8
Pale vs. yellow
Projection neurons
48
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49
ey-Gal4, dll-Gal4 gtGFP c699-Gal4 ap-lacZ
50
Comparing R7 vs. R8
R8
R7
R8
R7
51
Comparing Blue vs. Green
R8
R7
R8
R8
R7
R7
52
High level processing neuron
R8
R7
R8
R7
53
High level processing neuron
R8
R7
R8
R8
R7
R7
54
Modulatory cells Interneurons?
55
Multi-columnar modulation
R8
R7
R8
R7
R8
R7
t-lacZ
56
A cell modulating long-projecting neurons
R8
R7
R8
R7
57
Reinhard Wolf
Satoko Yamaguchi
Martin Heisenbergs lab
58
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59
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60
Optomotor response relies on contrast
1.2
1
0.8
0.6
0.4
0.2
0
0
50
100
150
200
250
-0.2
Green 175
(n23)
Blue intensity
61
Novelty paradigm
2min 30sec
30sec
62
G175 vs B136 (iso-luminance)
plt0.01 n62
63
Fly disco according to Reinhard Wolf
64
Mathias Wernet Esteban Mazzoni Tamara
Mikeladze-Dvali Daniela Pistillo Daniel
Vasiliauskas Javier Morante Satoko Yamaguchi Arzu
Celik Collaborations Steve Cohen Tiffany
Cook Thomas Labhart Mike OConnor Jessica
Treisman Reinhard Wolf
65
I was gratified to be able to answer promptly.
I said I dont know.

• Mark Twain

66
Appearance of UV, Blue, Green and Red pigments
Red
Green
Blue
UV
67
Chic
k
GF
R
e
d
Human
Human
Chic
k
G
r
e
e
n
GF1
GF2
Vertebrates
Human
Chic
k
R
o
d
s
GF
ZF
GF
B
l
u
e
Chic
k
Chic
k
U
V

Human
GF
R
h
6
G
r
e
e
n


R
e
d
Invertebrates
R
h
1
R
h
2
R
h
3
U
V

R
h
4

B
l
u
e
R
h
5
68
Open rhabdome vs. fused rhabdome
Flies
Bees
69
Optical properties of the insect eye
Bees Fused rhabdome
Flies Open rhabdome
70
Neural superposition
RETINA
n
LAMINA
h
A
B
C
D
E
H
B
C
F
G
A
D
E
4
I
J
F
G
H
1
6
5
2
3
J
3
4
I
4
5
2
L
M
5
K
2
6
1
6
K
L
M
1
1
6
N
O
N
O
2
5
Q
P
R
4
Q
S
T
P
R
3
S
T
Adapted from The physiology of insects,
Rocktstein M. et al. (1974)
71
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72
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