Title: BioCheck
1 BioCheck Powder Screening Test Kit
2Bioterrorism Renewed Threats
3 1 Real Threat 1,000 False Alarms
Powdered Sugar Flour
Cornstarch
Cosmetics
Drywall Dust
4 Needs of first responders
- Reliable results (low false positive and
negative) - Reasonable price
- Speed in obtaining results
- Ease of use
- Long shelf life robust
5Limitations of Existing Field Tests
- Require Instrumentation ()
- Different tests Needed for each agent (Ricin,
Anthrax, etc.) () - Complex to use
- Not easily scalable
- Short shelf life not robust
- False positive Panic!
6Needed
Simple, reliable, disposable, inexpensive, test
to quickly rule out most of the substances
that the public suspects might be bio-terror
agents
7 BioCheck Powder Screening Test Kit
- Indicates the presence or absence of protein in
suspicious powders through a simple color change.
- A simple chemical test for protein a change to
purple in the test tube indicates a possible
biothreat. - pH test provides additional information
(bioterrorist materials are more likely to be of
physiological (neutral) pH.
8BioCheck Powder Screening Test Kit
BioCheck simultaneously screens for the
bio-terror agents anthrax, ricin and botulinum
toxin in 10 minutes or less.
9BioCheck Kit Protocol
10BioCheck Protocol Step by Step
Confirm that product has not passed
expiration date (on front of box) before using.
11BioCheck Protocol Step by Step
Tubes and swabs should be at or near room
temperature before deployment
12BioCheck Protocol Step by Step
Do not confuse the Protein Detection swab
(used first to collect and test the sample) with
the Protein Control swab (used as a positive
control to confirm a negative result for
protein).
13BioCheck Protocol Step by Step
The pH test does not provide an answer to
the question Is this a possible bioterror
agent? (This is the function of the protein
test). Rather, it provides additional information
about the substance being tested
14BioCheck Protocol Step by Step
Be sure to read the result within 15
minutes of transferring the sample to the tube.
Warmer temperatures will cause the reactions to
proceed more quickly lower temperatures will
slow the reaction rates.
15BioCheck Protocol Step by Step
NOTE A positive is the appearance of a purple
color in the solution (not just on the swab or on
the powder sample) NOTE A positive (purple
color in the Protein test solution) does not
necessarily indicate the presence of a bioterror
agent merely the need for further testing. In
actual 911 calls, 87 of substances were negative
for protein.
16BioCheck Protocol Step by Step
If the Positive Control Swab does NOT cause a
negative protein test to turn positive (purple)
repeat testing with another BioCheck Kit or test
with a different methodology
17 BioCheck Result Summary Chart
The pH test is advisory high and low pH
materials are less likely to be biological in
origin. The protein result determines the proper
course of action, no matter what the pH result
Protein result Control result pH result Conclusion
Positive n/a Any Positive protein is present
Negative Positive Any Negative Follow local protocol
Negative Negative Any Invalid Test Result
18Examples of Positive and Negative Substances
(Protein)
- Protein Negative
- Dangerous chemical agents
- OR
- Sugar (powdered)
- Plaster dust
- Salt
- Corn starch
- Dry wall dust
- Protein Positive
- Biological warfare agents
- OR
- Yeast
- Pancake mix
- Corn meal
19Proven in the Field
- In more than 50 911 calls in 2002, more than 85
of substances tested were NEGATIVE for protein
ruling out a bio-terror agent. - The product has been successfully tested against
Anthrax and simulants - Hundreds of first responder agencies using
BioCheck nationwide the kit has been
incorporated into SOPs for responding to
suspicious powder calls.
20Threat Assessment Visual Assessment
PID Screening
Example of Field SOP
Radiological Screening
Chem Warfare Agent Screening
If warranted
20/20 Protein-pH Test
If NEG Protein-Hi/Lo PH
Sensor IR Screening
If POS Protein-Neutral pH
Prime Alert Microbe Test
If POSITIVE
Tetracore Strips (5 tests) If Airborne use
Mesosystems Air Sampler
If POS Notify SP Ops, MPD FBI
Prepare sample for Lab (SWAB) Bag Item for
evidence (Triple bag)
Do Not Perform Site/Evidence Decon until
Investigation is complete
Any POS indication shall be reconfirmed by a
minimum of two (2) methods no samples are
confirmed positive without laboratory
confirmation!
21For more information Ph 866-572-2020 24 hour
202 669-5189 www.biocheckinfo.com