ncRNA

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ncRNA

• Jeremy Logue

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The Expanding Repertoire of RNA
Adapted from Soares and Valcarcel (2006) EMBO
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The Expanding World of Non-coding RNAs
Adapted from Huttenhofer, A et al. (2005) Trends
in Genetics
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(No Transcript)
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HAR1-associated transcripts in genomic context
Of the 49 HARS identified (Human Accelerated
Regions), 96 are in non-coding regions.
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Predicted RNA secondary structure for HAR1F
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Expression of HAR1F and HAR1R in the developing
neocortex
Cajal-Retzius cells establish early neuronal
circuitry in the developing brain
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Expression of HAR1F in other parts of the
developing brain
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What does this all mean?
Fill in the blank
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X - Inactivation
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Dosage Compensation
Adapted from Straub and Becker (2007) Nature
Reviews Genetics
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Imprinted Versus Random X-inactivation
Adapted from Avnir and Heard (2001) Nature
Reviews Genetics
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The X-inactivation Center
Adapted from Avnir and Heard (2001) Nature
Reviews Genetics
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Xist Transcription in Embryonic Stem Cells
Differentiation
48 hrs.
Adapted from Avnir and Heard (2001) Nature
Reviews Genetics
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Potential Roles for Xist Antisense Transcription
Adapted from Avnir and Heard (2001) Nature
Reviews Genetics
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What does a silenced X look like?
Adapted from Chow, JC et al. (2005) Annu. Rev.
Genomic Hum. Genet.
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Co-localization and 3D - Microscopy
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How confident are we that these co-localize?
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Problems Associated with Co-localization
Resolution ?/(2NA) z-axis problem
Figure 1G from Chaumeil, J. et al. (2007) Gen Dev
Calculating a cross-correlation can also aid in
determining co-localization
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3D - Microscopy