The Incredible, Edible Soy

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The Incredible, Edible Soy

• By Chris Koscielniak, Dan Gardner and Sarah Masih
• The Isoflavone Team

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What are Isoflavones?

• Phytoestrogens (greek)
• Phyto-plant. Plant that carries out estrogen
  like activities.
• Isoflavones have received a great deal of
  research, especially for possible cancer and
  heart disease-preventive properties
• They take out free radicals in the body.

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Where can you find it?

• All the soyfoods in the following list are
  excellent sources of isoflavones, providing a
  range of 30 - 50 milligrams per serving.
• Roasted soy nuts (1 ounce)
• Soy flour (1/2 cup)
• Soy grits (1/4 cup)
• Textured soy protein (1/2 cup, cooked)
• Yellow, green vegetable or black soybeans (1/2
  cup, cooked)
• Regular soymilk (1 cup)
• Tempeh (1/2 cup)
• Tofu (1/2 cup)
• http//soyfoods.com/nutrition/isoflavone.htmlanch
  or115079

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POSITIVE

• Increases estrogen by stimulating the ovaries.
• This can lead to an easier menopause and lower
  the risk of breast cancer.
• Or lower prostate cancer with men.
• It may help reduce the risk of osteoporosis
• Reduce risk of atherosclerosis and other
  cardiovascular dieases.

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Negative Side Effects

• Suppression of thyroid
• May decrease lymphocytes in blood.
• We dont know effects on pregnancy and breast
  feeding
• Age limitations?

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Properties of Genistien

• Formula C15H10O5
• Formula mass 270.24
• Description odorless yellow-white powder
• Melting point 297-298ºC
• Insoluble in water

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Genistein

• Metabolised into p-ethylphenol
• Lacks oestrogenic activity
• Effects may be caused by blocking estrogen
  receptors in the brain from active chemicals

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Properties of Daidzein

• Formula C15H10O4
• Formula mass 254.24
• Description almost white solid
• Melting point 297-298ºC
• Boiling point sublimes
• Insoluble in water

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Daidzein

• Metabolised into equol and O-dma
• Does exhibit oestrogenic activity
• About one third of humans cannot metabolise it

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Properties of Coumestrol

• Formula C15H8O5
• Formula mass 268.23
• Description odorless solid
• Melting point 385ºC
• Insoluble in water

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Coumestrol

• Coumestrol was found in an osteoporosis study, to
  help suppress bone resorption
• Also has phytoestrogenic properties
• May work alongside Daidzein and Genistein in bone
  protection

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So Far

• We have attempted extraction of isoflavones from
• Natures Life Super-Green Pro-96 soy protein,
  it was inconclusive
• Arrowhead Mills Soy flour, and dry soybeans
• Soy milk

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Procedure

• 1-3g of sample (depending on which we used)
  placed into Erlenmeyer flask and 40mL of 80
  (vol/vol) methanol in water was added.
• The flask was covered with parafilm and/or tin
  foil and heated in a water bath with magnetic
  stirrer at approximately 65ºC for 2 hrs.

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More Procedure

• The flask was then cooled to room temperature and
  3 mL of 2 M NaOH was added
• This soln was stirred for an additional ten
  minutes at room temperature.
• One mL of Glacial Acetic acid was then added

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Even More Procedure

• The resting solution was then filtered through
  Whatmans No. 40? Filter paper into a beaker
• Five mL of this filtrate was then mixed with 4 mL
  of water and 1 mL of the 80 (vol/vol) methanol
  in water
• We then ran this final product through a GC-MS at
  varied settings

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Whats next?

• How do soy preperations affect isoflavone
  content?
• Which products have high isoflavone content?
• Effects of Isoflavones along side coumestrol
• Further properties of coumestrol

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Thanks for viewing!!!

• For more information please visit our website
  http//www.stfrancis.edu/ns/diab/CRT/CRT.html
• Thanks for your time!
• Chris Koscielniak, Dan Gardner, Sarah Masih