Zebrafish Jeopardy

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Zebrafish Jeopardy
General Characteristics Cell Movements Signaling and Pathways Developmental Stages Experiments
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General Characteristics for 100

• Name this structure
• What is Blastodisc?

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Cell Movements for 100

• During the gastrula stage, this happens around
  the whole embryonic margin.
• What is involution?

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Signaling and Pathways for 100

• The nodal signals are present in zebrafish.
• What are squint and cyclops?

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Developmental Stages for 100

• The Mid Blastula Transition (MBT) occurs during
  this stage.
• What is the Blastula stage?

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Experiments for 100

• Zebrafish are a good model organism for this
  genetic screen.
• What is forward genetic screen

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General Characteristics for 200

• The zebrafish egg shell.
• What is the chorion?

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Cell Movements for 200

• Cells that involute first and last give rise to
  these structures.
• What is anterior structures and posterior
  structures respectively?

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Signaling and Pathways for 200

• Where Nodal signal squint begin to be expressed.
• What is the Dorsal Yolk Syncytial Layer?

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Developmental Stages for 200

• Zygotic transcription begins during this stage.
• What is the Blastula stage (MBT)?

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Experiments for 200

• This protein allows cells to be visualized in
  vivo.
• What is Green Fluorescent Protein (GFP)?

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General Characteristics for 300

• The Zebrafish embryo undergoes this kind of
  cleavage.
• What is Meroblastic Discoidal?

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Cell Movements for 300

• The embryonic cells that undergo epiboly.
• What are all cells? (not just ectoderm)

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Signaling and Pathways for 300

• The Nieuwkoop needs these signals to induce the
  organizer.
• What are Beta-Catenin and Nodal?

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Developmental Stages for 300

• When a fate map can be made.
• What is Late Blastula? (after cell mixing)

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Experiments for 300

• Transplanting a second organizer on the ventral
  side causes this phenomenon.
• What is double axis formation?

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General Characteristics for 400
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• Name this structure
• What is Yolk Syncytial Layer?

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Cell Movements for 400

• Involuting cells give rise to these germ
  layer(s).
• What are Mesoderm and Endoderm?

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Signaling and Pathways for 400

• BMP is inhibited on the dorsal side by these
  molecules.
• What are chordin and noggin?

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Developmental Stages for 400

• Epiboly begins in this stage.
• What is the Blastula stage?

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Experiments for 400

• This type of experiment occurs when Nodal
  inhibitor Activin is added to the zebrafish
  embryo there is a decrease in mesoderm.
• What is loss of function?

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General Characteristics for 500

• The yolk free area of a zebrafish embryo.
• What is Blastodisc?

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Cell Movements for 500

• Anterior-Posterior axis is formed by this cell
  movement of this cell layer?
• What are mesendoderm and ectoderm undergoing
  convergent extension?

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Signaling and Pathways for 500

• Notal signaling is required for this embryonic
  layer formation.
• What are Mesoderm and Endoderm?

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Developmental Stages for 500

• Dorsal convergence to form the shield occurs
  during this stage.
• What is the Gastrula stage?

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Experiments for 500

• A cyclops squint double mutation would create
  this phenotype.
• What is lack of mesoderm and endoderm?

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