Chapter 8- Antigen Processing and Presentation

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Chapter 8-Antigen Processing and Presentation
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Where were going

• Definitions- processing, presentation, MHC
  restriction- evidence for the latter.
• Tell the story of antigen presentation, beginning
  with the antigen until its on the surface of the
  cell- well be learning some cell biology in the
  process.

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• Processing Breaking up the antigen into pieces
  that fit into the MHC I or II groove.
• Presentation putting the peptides on the groove.
• Again, endogenous and exogenous antigens

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MHC Restriction

• Tough concept
• Fundamentally, T cells only recognize Ag
  presented by the MHCs that they were trained to
  recognize in the thymus.

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These do exogenous Ag presentation
Th cells
Uptake of 3Hthymidine
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For this experiment, the Ag thats presented is
not seen- wrong MHC!
These are seen as self
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Non-pros get activated by inflammation!
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LMPs- induced by infection, make production of
MHC I peptides more likely
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Tapasin, Calreticulin, and Calnexin are
Chaperones- stabilize, help fold, put the peptide
on the class I MHC
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The text has descriptions of each step!
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HLA-DM- non-classical MHC- catalyst for exchange
of CLIP for peptide
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What to know

• Definitions- processing, presentation, MHC
  restriction- evidence for the latter.
• Tell the story of antigen presentation, beginning
  with the antigen until its on the surface of the
  cell-
• MHCI- roles of ubiquitin, LMPs, proteasome, TAP,
  tapasin, calreticulin, calnexin (chaperones)
• MHC II- roles of invariant chain, CLIP, MHC DM,
  endocytic processing.