Calcium-butyrate SINTOBUTYL Product and specifications

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Calcium-butyrateSINTOBUTYLProduct and
specifications
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Calcium-butyrate Product and specifications

• Sintobutyl

• - Chemically pure calciumbutyrate (slight surplus
  of Ca) fine powder
• Doesnt smell if not touched with humid surfaces
  (hands, ...).
• May partly dissociate in contact with humidity
  and release unpleasant smell
• Min. 76.6 butyrate-ANION and approximately 17
  Calcium

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Sintobutyl
Coating incapsulates the active compound
(Ca-butyrate) ?

Fat components from vegetable origin ( Very good
nutritional properties)

• Fatty acids with high melting point
• Hydrogenated fatty acids

C14 and lower () 1 C16 palmitic acid () 45
C18 stearic acid () 53 C18oleic acid
() 0.5 C20 and higher 0.4
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Sintobutyl (calcium butyrate) and Antibiotic
Growth Promoters
How Sintobutyl ranks between other claimed
alternatives ?
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Sintobutyl versus Other Gut Modifiers
Gut
Flora Stabilisers .
Are they all the same ?
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More Feed Additives claim, support BUTYRATE
effects
Protection and regeneration of intestinal villi
(mucosa integrity)
Non Digestible Oligosaccharides
Bacterial shift (LAB, Bifido)
F.O.S. (inuline, profeed) M.O.S. (yeast
cell walls)
Fermentation to VFA and more Butyric (Megasphera
LA -gt BA)
Selective bacterial inhibition at intestinal,
ceacal level Coli,
Clostridium, Salmonella
Butyrate (butyric acid)
Immuno-stimulant
Stimulation of enzyme synthesis
Bacteria attachment (lectins)
Some induce BA production ()
Herbal Extracts
Stimulation of immunity
Zootechical effects FI, DWG, FCR, Egg Shell
strength
Part is converted at intestinal level to Butyric
MCFAs
So, why not adding butyrate (Sintobutyl) directly
!!??!!
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Mode of action of PROGUT(Vuorenmaa J Vaahtovua
J. (2006)
PROGUT Hydrolysed brewery yeast product
Prebiotic effects Yeast Cell
Wall effects are claimed

• Stabilize gut flora
• Support natural immunity
• Prevent attachment of E.Coli, Salmonella to mucus

Increases the MBI with piglets and poultry !!!
Bifidobacteria butyric acid producing bacteria
MICROBIAL BALANCE INDEX --------------------
-------------------------------------------
Enteric group bacteria bacteroides
Significant correlation with DWG and FCR
Means more Butyrate in the gut, so just give
straight Sintobutyl !!!
MBI
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• Comparative mode of action and benefits
  of ABGP
  ButyProl Coated MOS FOS MCFA
  Benzoic E.O.
• 
  Butical Acids YCWs
  Acid Herbs

• Gut integrity
• Enzyme synthesis
• Immunity
• Microbial Shift (beneficial)
• Metabolic activity flora
• Inhibition - Coli
• - Clostridium
• - Salmonella

- / - -
/- - - /- - -
/- - - - -
- - - - - -
- - /- - -
- - /- - -
- - /-
colon ?
?
- - - - colon
? ? ? indirect
direct indirect colon
direct ? / - -
- - - -
- - ? - ? - ? -
- / - -
- ? - ?

• Digestibility of nutrients
• Absorption
• FCR
• Intestinal disorders
• Pathogens
• Litter quality

- - - - - ?
? -
- - -/ -/ (c) - ?
- ? - -
? - - -
? ?

- - /
?-
- /
- - - - -/
- ? /- -/
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• Comparative mode of action and benefits
  of ABGP
  ButyProl Probiotics
• 
  Butical

• Gut integrity
• Enzyme synthesis
• Immunity
• Microbial Shift (beneficial)
• Metabolic activity flora
• Inhibition - Coli
• - Clostridium
• - Salmonella

- / /-
- - - - -
- /-

- - indirect
direct indirect / - -
-/ - - /
--/

• Digestibility of nutrients
• Absorption
• FCR
• Intestinal disorders
• Pathogens
• Litter quality

- -
- - -
indirect
-- / - - /
--/ - /
- -
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Sintobutyl versus Aromabiotic (medium chain fatty
acids) in rabbits
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Totally untrue !!!!!!!!!!! Pure Marketing
!!!!! Sept 06 so far limited use in 2-3
countries of EU-25 and on the way back (fashion)
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Ca-butyrate (Sintobutyl) versus MCFAs
CLAIM-ACTIVITY MCFA
Calciumbutyrate
MCFA soluble both in water and lipids, which
would influence the speed of penetration of the
product through the bacterial cell wall.
Molecules have medium length C10-C14 and may be
quite big to penetrate.
Bacterial Inhibition E. Coli Clostridium Vil
li integrity Performance (dwg,fcr)
Butyric acid is also soluble in water and in fat,
both lipofylic and hydrofylic. On top the
molecule is smaller (C-4), which normally could
be easier to penetrate.
1.0-1.5 log reduction (10-15 days) at 0.2 -
MIC 0.5 !!
1.0 -1.2 log reduction (25-50 mmol) J .
Decuyper 2003-U.G.
MCFA would inhibit lipase production by bacteria,
which seems needed for bacteria to attach to the
gutwall. So attachment may be hindered and
bacteria lessivate !? INDIRECT effect
Calciumbutyrate has a DIRECT inhibition effect at
pH6 on all pathogenic clostridium strains
(A.Decostere). Inhibtion of anaerobes by 0.5 log
(J. Decuyper)
MCFA would Increase villi length and vili/crypt
ratio, but this is again INDIRECT (as more
additives may do by fermentation or
transformation to butyric acid)
Butyrate is the FUEL (nutrient) of the
gutwallcell (villi), having a DIRECT effect on
the gutwall integrity and regeneration. Besides
systemic effect, also the topical effect (local)
Effects seems limited, nihil to negative (on feed
intake). Few data !
Significant Positive Effect (higher feed intake
-attractant). Full data base !
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Butyrate MCFAs similarities differences !?
Property, advantage MCFAs
Butyrate (butyric
acid) Specific Characteristics
Chain length caproic C6,
Caprylic C-8, Capric C10 C4 (longest
SCFA) pK-value 4.88
4.89 4.89
4.86 Solubility
fat and water soluble
fat and water soluble ME
26.6 kJ/gr
17.4 kJ/gr Human
application as functional lipid
(slimming) as anti-cancer
(colon) Absorption
enhancing (paracellular permeability)
enhancing (idem emulsifyer) Bacterial
Inhibition mg/ml E. Coli
gt 5 2
5
lt 5.0 (UG-AF) Salmonella
gt 5 3
gt 5
lt 5.0 (UG-VF) Clostridium
gt 5 1-2
1 4-16
(UG-VF)
C-12 0.1-0.2
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Butyrate MCFAs similarities differences !?
Property, advantage
MCFAs Butyrate
(butyric acid) Specific Characteristics Ketosis
effects (negative !!) Risk C6-C8 in
neonatals (piglets) not (only in
ruminants) Association with C-4, VFA
Antagonist (drop in C-4, VFA)
Synergistic ( or gt) gtCompatible with FOS,YCW,
NOT
YES Some
Probiotics, .... Risk of lipolysis in feed
Real
NOT
(lipase) Required antimicrobial 3-5
gr/l pure, stomach, gut concentration

FI piglet 300 g/d gt
10-17 kg per ton pure
X 2 20-34 kg per ton
of feed 0.5-3.0
kg/ton Accumulation in fat
at high dosages (4 )
NOT
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MCFAs Butyrate antagonist !!!!! Conflicting
Worrying ???

• J. Goris (2006), ILVO C8 or caprylic acid
  changes fermentation profile in caecum of piglets
  increase lactate, decrease butyrate and
  propionate (reversible)
• F.Boyen Pasmans (2006) Effect on salmonella
  invasion of caprylic and caproic acid butryic
  acid is antagonistic. Butyric alone decreases
  invasion !
• Leeson Antongiovanni (2005) Triglycerides of
  mcfa butyric less effective in broilers than
  Triglycerides of butyric acid alone.
• Van Oeckel (2005) - ILVO Piglets trial
  performance of combination of mcfa-product
  butyrate less good than mcfa, butyrate, but also
  than negative control.
• Maertens L (2006) ILVO Rabbits mcfa-product
  decreased significantly VFAs and Butyric
  concentration in caecum. Unbalancing cecal flora
  !!!

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Salmonella invasion through epithelial cells of
broiler chickens after treatment with butyric
acid or MCFA (caproic acid)
IN VITRO !!
MCFA seem to decrease invasion at least to the
same extent as butyric acid but at lower
concentrations F. Van Immerseel et All (2004)
Appl. Environ.Microbiol. 6/2004 3582-3587

• In Vitro data what happens in intestine with
  additive ? How much caproic acid is liberated
  from coconut-oil ?
• Related only to eventual accumulation in liver
  and spleen, not in caecum (see in vivo test)

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Salmonella in different organs of broiler
chickens after treatment with butyric acid or
MCFA (caproic acid)
Log cfu/gr
IN VIVO !!
-1.66 log
-2.2 log
a b a a a a
a b a b a a
6-30 kg MCFA-product ??
3 kg Butical 60
Inoculation with 10exp3 Salmonella Enteritidis on
day 5, counts on day 8 (Van Immerseel -
University Ghent)
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Composition of a commercial MCFA product
Method saponification
BF3/MeOH (hot) BF3/MEOH
(cold)
BF3/MeOH (hot) Fatty Acid rel
g/100 gram rel g/100 gram
rel g/100 gram Caproic C60
8.1 5 8.6
5 6.9
3 Caprylic C80 62.1 32
60.8 30
59.6 23 Capric C100 29.4
15 30.2 14
33.0 12 Undecanoic C110
0.2 0 0.2
0 0.3
0 Lauric C 120 0.2 0
0.2 0
0.2 0
53 49
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Sample from Belgium 2004 Independent
industrial laboratory, analysis in 2005