Cloning Rab5WT and Rab5 Mutants to Study the Endocytic Pathway Involving TGF-? Receptor and Rin1

1
Cloning Rab5WT and Rab5 Mutants to Study the
Endocytic Pathway Involving TGF-? Receptor and
Rin1

• Min Kang
• Colicelli Lab, UCLA

2

• Early Endosome Trafficking is Required
• for RTK Down-Regulation

Growth factors
RTK
RTK
RAS
Adaptor
GEFs
GAPs
GTP
Rab5
Recycling endosome
Other Effectors
Early endosome
RAF
PI3K
RIN1
MEK
PIP3
ABL1/2
ERK
AKT
Late endosome/ lysosome
Cell growth
Cell motility
Cell survival
3

• RIN1 Regulates TGF? Signaling through Vesicles
  Trafficking

Clathrin- coated pit
Lipid raft
caveolae
CAV1
P
P
RIN1
2
1
ABL
RAB5
3
Early Endosome
Caveolar vesicle
RAB5
SMAD2
SARA
SMAD7
SMURF2
P
SMAD2
TGF?R downregulation
TGF? signaling
4
Rab5 clones
Rab5WT
Rab5Q79L (DA)
Rab5S34N(DN)
Mlu
Mlu
EcoRI
XhoI
Nhe
Nhe
SalI
M4-IRES-GFP
M4-IRES-GFP
M4-IRES-GFP