Autoclave

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Autoclave

• By Carolina Gallango-Brun
• Sana Khan
• Vedrana Sahovic

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Definition

• Autoclave is a pressurized device designed to
  heat aqueous solutions above their boiling point
  at normal atmospheric pressure to achieve
  sterilization

Fig. 1. Autoclave machine
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Brief History

• 1450 B.C.E. Moses
• 460-377 B.C.E. Hippocrates
• Lazzaro Spallanzani
• Pasteur
• Denis Papin

Fig. 2. Papins steam cooker
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20th Century

• It was finalized by Charles Chamberland in 1987
• Colleague of Pasteur
• Building up on Papins marmite
• From culinary to medicine

Fig. 3. Charles Chamberland 1851-1908
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How Des it Works?

• Liquid H2O cannot be heated above 100C in an
  open vessel
• At 100C boils
• H2O heated in a sealed vessel
• Pressure rises
• Boiling point of H2O is raised

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The Process

• Air removal
• Downward displacement
• Steam pulsing
• Vacuums pumps
• Superatmospheric
• Subatmospheric

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Autoclave Usage

• Microbiology
• Medicine
• Body piercing
• Veterinary science
• Dentistry
• Podiatry
• Metallurgy

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Video Clip
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References

• http//en.wikipedia.org/wiki/Autoclave
• http//138.23.152.128/protocols/autoclave20operat
  ion.pdf