CYTOTOXICITY EFFECT OF RESVERATROL OLIGOMERS AND THEIR DERIVATIVES AGAINST HUMAN CANCER CELL LINES

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CYTOTOXICITY EFFECT OF RESVERATROL OLIGOMERS AND
THEIR DERIVATIVES AGAINST HUMAN CANCER CELL LINES

• Sri Atuna, Nurfina Aznama, Retno Arianingruma,
  Takaya Yb, Niwa Masatakeb

a Department Chemistry education, Universitas
Negeri Yogyakarta, Karangmalang, Depok, Sleman,
Yogyakarta, 55281 b Faculty of Pharmacy, Meijo
University, Tempaku, Nagoya, Japan
METHODE OF RESEARCH
Sample milled dried stem bark
Hopea is one the main genus of Dipterocarpaceae,
consisting of approximately 100 species and
widely distributed in Indonesia specially in
Kalimantan The local name is cengal, merawan
hitam or pengarawan. The plant ussually can be
used as material building, plywood etc. This
family of plant is known to produce a variety of
resveratrol oligomer These structures are very
interesting and showed interesting biological
activity, such as antibacterial, anticancer,
antihepatotoxic, and anti-HIV
Extraction by organic solvent acetone or
methanol
EXTRACT
By fractionated (VLC)
Fr A
Fr B
Fr D
Fr C
Repeated Chromatography
elusidasion structure by Spectroscophy
methode (UV, FT-IR, NMR, MS)
Pure compounds S-1, S-2, etc
Methylation Acethylation
BIOACTIVE COMPOUNDS
STRUCTURE MOLECULE
DERIVATIVES COMPOUNDS
Isolated compounds from H. odorata (3.8 Kg)
Isolated compounds from H. mengarawan (5 Kg)
Isolated compounds from H. nigra (3.5 Kg)
Vaticanol G (70 mg)
Hopeaphenol (1500 mg)
Heimiol A (200 mg)
Balanocarpol (300 mg)
Balanocarpol (300 mg)
vaticanol G (200 mg)
Ampelopsin H (250 mg)
Hemlesyanol C (120 mg)
vaticanol B (200 mg)
Methode identification structure by modern
spectroscophy
Spectrum UV and IR of Vaticanol B
Spectrum 1H-1H COSY NMR of vaticanol B
Spectrum 1H and 13C NMR of vaticanol B
Cytotoxicity test by Raji cell lines
Cytotoxicity test by Hela S3 cell lines
Hela S3 cell lines before experiment Hela
S3 cell lines after experiment
Raji cell lines before experiment Raji
cell lines after experiment
Table 1. The cytotoxic effects of resveratrol
oligomers and their derivatives in
human cancer cell lines
Conclusion In this paper we conclusion that
isolated compound from steam bark of H.
mengarawan, H.odorata and H. nigra have
cytotoxic effect against Raji, HeLa-S3, and
Myeloma cell lines. Vaticanol B (4) and
ampelopsin H (6) give the highest cytotoxic
effect against HeLa-S3 and Raji, while the
derivative resveratrol, methyl and acethyl
balanocarpol and hopheaphenol showed not
active. Acknowledgements This work has been
supported by competitive grant (Insentif Riset
Dasar, Ristek- 2008), Ministry Reseach and
Technology, Republic of Indonesia. The authors
are grateful to the experimental Garden in
Carita, Pandeglang, Banten, Indonesia and
Herbarium Bogoriensis for supported the sample
and identification of the plant specimen.
No Sample LC50 µg/mL LC50 µg/mL LC50 µg/mL
Hela- S3 Raji Myeloma
1 2 3 4 5 6 7 8 9 10 11 12 Balanocarpol Heimiol A Vaticanol G Vaticanol B Hopeaphenol Ampelopsin H Hemlesyanol Deca-methyl-O-hopeaphenol Deca-acethyl-hopeaphenol Penta-methyl-O-balanocarpol Hexa-acethyl-balanocarpol Doxorobicin (positif control) 692.78 gt 1000 gt 1000 92.81 gt 1000 129.71 557.44 gt 1000 gt 1000 gt 1000 594,29 96,87 235.29 gt 1000 gt 1000 34.45 781.49 34.69 292.14 gt 1000 gt 1000 gt 1000 594,29 242,10 197.61 gt 1000 gt 1000 250,15 119.62 756.43 gt 1000 gt 1000 533.85 298,06
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