Cultivation of anaerobes

1
Lab 7

• Cultivation of anaerobes
• Motility determination

2
Motility Test
3
CULTIVATION OF ANAEROBES
4

5
OXYGEN NEEDS GROWTH PATTERNS
6
GROWTH PATTERNS IN THIOGLYCOLATE MEDIUM
7
Oxygen Requirements as Determined in Fluid
Thioglycolate Medium
8
INOCULATION OF TRYPTONE GLUCOSSE YEAST EXTRACT
AGAR (TGYA)
9
INOCULATION OF TGYA plates
S. aureus
E. coli
E. coli
C. taeniosporum
10
O2 removed by Chemical Rxn H2O
Resazurin
GASPAK SYSTEM
11
GASPAK
12
ANAEROBICCHAMBER

• Abaerobic Chamber
• O2 REPLACED W/ INERT GAS

13

• Ex. 18 Motility
• Tube method, Ex. 5-28 Inoculate semisolid agar
  tube with the needle Microorganisms Proteus
  vulgaris (All studnetsTeam A) Bacillus subtilis
  (Half students students Team B) E. coli (half
  students Team B)
• 2) Plate Method Inoculate by Stabbing the
  medium (SA), the 3 strains In the same plate (2
  plates/ Table)
• Ex. 21 Cultivation of anaerobes (two teams) Ex.
  2-7 2-8
• Microorganisms Clostridium esporogenes,
  Clostridium taeniosporum, Sthaphylococcus aureus,
  E. coli
• Inoculate
• TGYA Tubes, melted medium-. 1 Tube /organism
  (Total 4 tubes/team)
• TGYA Plates (Fig. 21.4) 1 plate / Team (4
  organism/plate)
• Fluid thioglycolate (FTM) medium 1 Tube/organism
  (4 tubes/ Team)
• Team A Incubate anaerobically in a GasPak
• Team B Incubate aerobically in incubator

14
Unknowns

• Do a GRAM stain of each
• Write colony morphology characteristics
  (including pigments)
• Finish your dichotomous key
• Link for Dichotomous keys
• http//sites.google.com/site/scienceprofonline/vir
  tualmicrobiologyclasslaboratory233
• Achieve the 2 colony types isolation