?????????(CZE)

1
??????????????

• ?????????(CZE)
• capillary zone electrophoresis
• ?????????(CZE)
• capillary gel electrophoresis
• ???????????
• micelle electrokinetic capillary electrophoresis
• ?????????(CIEF)
• capillary isoelectric focusing
• ?????????
• capillary isotachophoresis

high performance capillary electrophoresis,HPCE
??????????????
separate types of HPCE
2
????
??????,??????? ????????,????????? ??????????
3
?????????capillary zone electrophoresis ,CZE

• ??????????????????????
• ??????????????,???????
• ?????????,??????,???????

?????????????????? gt????,??????????,??????,?
???????,????????????????? ????????????
4
????????? capillary gel electrophoresis ,CGE
???????????????????? ??????,????????,?????
???????????????,??????,??????
????DNA????/??????????,CZE??????,??CGE???????,DAN?
??????? ???????,????,??????
????????????????????????????????????????
5
?? ?????????(MECC ,MEKC)micellar electrokinetic
capillary chromatography,MEKC
1.???????????????,?????????,?????????????????
????????,????????
6
2. ????????????,????? gt ??? ,???????????????
3.???????????(??)???,???????????????,?????
4.?????????,???????????????
5.?????????????
7
????????? capillary isoelectric focusing,CIEF
1. ??????????????????????? 2.
???????????(?????????????????????????),???????(68
V)?,??????????????????pH?? 3.
???????????????????pH??,??????????,?????????????,?
???,????
8
4. ????????????????????????,??????????pH????,??
??,????,???????????
5. ?????????,?????NaOH?? 6.
?????????????????????? 7. ????CIEF???,???????
9
????????? capillary isotachophoresis ,CITP
1. ?????????????????????(?????)?????,???????????
????,????????? 2. ??????,???????????????????????
?????????????????,???????????????????,?????
10
capillary isotachophoresis ,CITP
3. ?????????,????????????(?µE),?????????????
??????,?????????1?2?3?4? ? ?
????????????2???????3?,???????,?????,???2??
2??????1??,????????? 4. ??????,???????
11
????????? capillary electroosmostic
chromatography ,CEC
?????????????????????,????????,???????????????
????????
12
?????
??? ?? generalization ??? ???????????? basic
theory of HPCE ??? ????????? high performance
capillary electrophoresis apparatus ???
???????????? separate types of HPCE ???
????? application and advances of HPCE
??