Title: Biocontrol bacteria and actinomycetes, evaluation of their secondary metabolites against fungal pathogens and insectpest of chickpea
1Biocontrol bacteria and actinomycetes, evaluation
of their secondary metabolites against fungal
pathogens and insectpest of chickpea
Meesala Sree
Vidya1 and S Gopalakrishnan2 1-Jawaharlal Nehru
Technological University, Hyderabad 2-
International Crops Research Institute for the
Semi Arid Tropics, Patancheru
Different bacterial isolates inhibiting fungal
pathogens.
- Introduction
- In the present investigation antagonistic and
entomopathogenic bacteria and actinomycetes
against important fungal pathogens Sclerotium
rolfsii, Fusarium oxysporum f. sp ciceri (FOC),
Rhizoctonia bataticola (3 strains, RB-6, -24 and
-115), Fusarium andiyazi (FM943), Fusarium
proliferatum (FM-242), Macrophomina phaseolina
(MP) and insectpest of chickpea, Helicoverpa
armigera, were studied. - It is well known that bacteria and actinomycetes
found most common in compost and rhizosphere
soils. Hence five different herbal vermicompost
such as Neem, Annona, Gliricidia, Jatropha,
Parthenium and rhizosphere soils from organic
fields were selected for isolating the microbes.
- Bacteria and actinomycetes isolated from above
sources were characterized for their antagonistic
activity against fugal pathogens and insect pests
of chickpea. Most potential cultures will be
further characterized for in-vitro PGP and
biocontrol traits.
FOC Vs SBI-20
Rb-6 Vs SBI-20
S. rolfsii Vs VBI-1
FM-943 Vs SBI-20
No of actinomycetes isolates antogonistic to
different fungal pathogens
SRI
Objective The main objective of the present
investigation is to isolate and identify the
active compounds responsible for the inhibition
of the fungal pathogens and H. armigera from the
potential antagonistic microbes and
entomopathogens
CONT
No of bacterial isolates antogonistic to
different fungal pathogens
Various fungal diseases of Chickpea
Fusarium wilt
Charcoal rot
Dry root rot
Botrytis gray mold
- Results and Discussion
- Out of 88 actinomycetes isolated, 55, 45, 58 and
37 isolates inhibited MP, Rb-6, Rb-24 and Rb-115
respectively 33, 36, 13 and 13 isolates
inhibited FOC, FM-242, FM-943 and S. rolfsii
respectively. - From 72 bacteria isolated, 28, 25, 29 and 10
isolates inhibited MP, Rb-6, Rb-24 and Rb-115
respectively 31, 31, 5 and 22 isolates inhibited
FOC, FM-242, FM-943 and S. rolfsii respectively. - The most promising isolates (20) were further
evaluated for their PGP and biocontrol traits.
The results indicated that xx, xx and xx were
shown positive for chitinase, protease, lipase,
respectively.
- Materials and methods
- Bacteria and actinomycetes were isolated from
five herbal vermicomposts and rhizosphere soil
from organic field by serial dilution method. - Antagonistic activity of the cultures were
evaluated by dual culture assay. - The promising antagonistic cultures were further
characterized for their PGP and biochemical
traits.
- Future work
- Purification of active compound (s) from the
culture filtrates. - Physiological characteristics of the promising
cultures. - Green house and field evaluation of the promising
cultures and their metabolites.
Corresponding author M. Sree Vidya, Email
vidya.meesala_at_gmail.com