Biocontrol bacteria and actinomycetes, evaluation of their secondary metabolites against fungal pathogens and insectpest of chickpea

1
Biocontrol bacteria and actinomycetes, evaluation
of their secondary metabolites against fungal
pathogens and insectpest of chickpea

Meesala Sree
Vidya1 and S Gopalakrishnan2 1-Jawaharlal Nehru
Technological University, Hyderabad 2-
International Crops Research Institute for the
Semi Arid Tropics, Patancheru
Different bacterial isolates inhibiting fungal
pathogens.

• Introduction
• In the present investigation antagonistic and
  entomopathogenic bacteria and actinomycetes
  against important fungal pathogens Sclerotium
  rolfsii, Fusarium oxysporum f. sp ciceri (FOC),
  Rhizoctonia bataticola (3 strains, RB-6, -24 and
  -115), Fusarium andiyazi (FM943), Fusarium
  proliferatum (FM-242), Macrophomina phaseolina
  (MP) and insectpest of chickpea, Helicoverpa
  armigera, were studied.
• It is well known that bacteria and actinomycetes
  found most common in compost and rhizosphere
  soils. Hence five different herbal vermicompost
  such as Neem, Annona, Gliricidia, Jatropha,
  Parthenium and rhizosphere soils from organic
  fields were selected for isolating the microbes.
  
• Bacteria and actinomycetes isolated from above
  sources were characterized for their antagonistic
  activity against fugal pathogens and insect pests
  of chickpea. Most potential cultures will be
  further characterized for in-vitro PGP and
  biocontrol traits.

FOC Vs SBI-20
Rb-6 Vs SBI-20
S. rolfsii Vs VBI-1
FM-943 Vs SBI-20
No of actinomycetes isolates antogonistic to
different fungal pathogens
SRI
Objective The main objective of the present
investigation is to isolate and identify the
active compounds responsible for the inhibition
of the fungal pathogens and H. armigera from the
potential antagonistic microbes and
entomopathogens
CONT
No of bacterial isolates antogonistic to
different fungal pathogens



Various fungal diseases of Chickpea
Fusarium wilt
Charcoal rot






Dry root rot
Botrytis gray mold

• Results and Discussion
• Out of 88 actinomycetes isolated, 55, 45, 58 and
  37 isolates inhibited MP, Rb-6, Rb-24 and Rb-115
  respectively 33, 36, 13 and 13 isolates
  inhibited FOC, FM-242, FM-943 and S. rolfsii
  respectively.
• From 72 bacteria isolated, 28, 25, 29 and 10
  isolates inhibited MP, Rb-6, Rb-24 and Rb-115
  respectively 31, 31, 5 and 22 isolates inhibited
  FOC, FM-242, FM-943 and S. rolfsii respectively.
• The most promising isolates (20) were further
  evaluated for their PGP and biocontrol traits.
  The results indicated that xx, xx and xx were
  shown positive for chitinase, protease, lipase,
  respectively.

• Materials and methods
• Bacteria and actinomycetes were isolated from
  five herbal vermicomposts and rhizosphere soil
  from organic field by serial dilution method.
• Antagonistic activity of the cultures were
  evaluated by dual culture assay.
• The promising antagonistic cultures were further
  characterized for their PGP and biochemical
  traits.

• Future work
• Purification of active compound (s) from the
  culture filtrates.
• Physiological characteristics of the promising
  cultures.
• Green house and field evaluation of the promising
  cultures and their metabolites.

Corresponding author M. Sree Vidya, Email
vidya.meesala_at_gmail.com