Bio Stuff

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Bio Stuff
I. Bloodborne pathogens II. Biohazards/biosafety
bio waste "look-alike" III. Sharps IV. IBC
(Institutional Biosafety Committee)
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Bloodborne Pathogens (BBPs)
I.
(This is not official BBP training, just a
general intro.)
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What Are Bloodborne Pathogens? (BBPs)

• Bloodborne pathogens are microorganisms (viruses,
  bacteria, fungi.... that are carried in blood and
  can cause disease in humans.

path feeling and often used to mean BAD
feeling. (pathos, pathology, psychopath,
sympathy...)
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Examples of BBPs

• Malaria
• Syphilis
• Brucellosis
• Hepatitis B (HBV)
• Human Immunodeficiency Virus (HIV)
• Many others, but focus is largely on HIV, HBV,
  and HCV lately.
• because.....

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Because . . .
(focus is largely on HIV/HBV/HCV lately)
There are no cures, not even effective
treatments, for HIV or Hepatitis B
C. And because Scientists and medical
professionals who work with human
products are at risk.
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Transmission of BBPs

• BBPs are transmitted through contact with
  infected human fluids such as
• Semen
• Vaginal secretions
• Cerebrospinal fluid
• Synovial fluid
• Pleural fluid
• Peritoneal fluid
• Amniotic fluid
• Saliva

Infected here means containing blood which is
infected.
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Intact Skin Provides a Barrier

• Unbroken skin forms an impervious barrier against
  bloodborne pathogens.

Broken Skin Does Not
Infection can enter through

• open sores
• cuts
• acne
• hangnail

• abrasions (scrapes)
• sunburn
• blister

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Mucous Membranes do not provide a barrier either.
Bloodborne pathogens can pass through mucous
membranes

• For example

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Special BBP training is required

• If you work with human products, you must have
  this training from REM or someone else trained to
  give it. Annually.
• Everyone, including those who have received BBP
  training, be aware of..

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Universal Precautions

• Assume that all human products are BBP
  contaminated and will make you SICK
• Always wear PPE in exposure situations.
• Call for trained help if necessary.

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Hygiene Rules

• If you are working in an area where there is
  reasonable likelihood of BBP exposure, you should
  never
• Eat
• Drink
• Smoke
• Apply cosmetics
• Handle contact lenses

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Biohazards, biosafety, bio waste , and
look-alike waste
II.
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Purdue Biological Safety Manual
Contains much info.
online at http//www.purdue.edu/rem/home/booklets/
bioman.pdf
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Biological Safety Manual
Explains...

• Classification of organisms
• Lab Biosafety Level criteria -- required work
  conditions/equipment
• Decon/Disposal
• Precautions

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At Purdue we use two category designations to
refer to bio waste

• Category 1 bio waste
• Category 2 bio waste, also called "look-alike"
  waste

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Category 1

• any items known, assumed, or suspected of being
  infectious to humans before treatment..

Human cells all the BBP stuff on slide 4 always
category 1
Category 1 also includes all Risk Group 2 and
higher human pathogens.
Cat 1 wastes must be treated (chemically or with
heat) before REM picks them up
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Category 2

• Category 2 everything else that looks like
  category 1 but does not fit the defintion. No
  treatment required.
• REM will pick up both cat 1 and cat 2, just make
  a phone call and complete a short form,
• certification form
• for removal of EITHER cat 1 or
  cat 2 waste

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III. Sharps
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Sharps

• Defined cut, puncture, abrade you.
• broken or other sharp glass
• sharp or broken plastic
• razor blades/scalpels
• needles, other sharp metal
• Do NOT put these in the regular trash baskets.
• REFER TO HANDOUT at this time

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Biohazard contaminated sharps

• Category 1 - collect in puncture proof container,
  autoclave-able container if autoclave is your
  treatment method.
• Category 2 - collect in puncture proof container
• Dispose as all bio and look-alike waste (call
  REM, fill out cert form).

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Chemical contaminated sharps

• Decontaminate.
• Collect blades/needles, sharp metal in puncture
  proof container.
• Collect sharp glass/plastic in cardboard box.
• Keep in lab, not on floor, not hall.
• Custodian will remove it (maybe).

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Disposal of clean sharps

• See handout guidance re
• Needles/blades -- puncture proof
• Broken glass. -- cardboard box
• also, not in handout
• It is OK (in Chemistry at least) to take it out
  to dumpster yourself.
• It is NOT OK to put it on the floor except in
  approved waste glass container.
• It is NOT OK to put it out in the corridor.

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IV. Institutional Biosafety Committee

• IBC oversight of biohazard and recombinant DNA
  work is the rule.
• Guidelines and requirements come from NIH, CDC,
  and University policy.
• Must have protocol approval

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Thank you.