Lentikats Biotechnology in Food Industry

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Lentikats Biotechnology in Food Industry
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Content

• Our aim
• What is Lentikats Biocatalyst
• Production of Lentikats Biocatalyst
• Benefits of Lentikats Biotechnology
• Lentikats Biocatalyst in Food Industry
• Our guarantees
• Our partners
• Contacts

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LentiKats a.s. in Food Industry

• LentiKats a.s. follows up on nine year of
  research and development of Lentikats
  Biotechnology.
• Our production know-how for commercial use of our
  Biocatalyst and for its industrial applications
  is patented internationally.
• Our main focus is in intensification of
  production processes that leads to
• higher process yields
• enhanced process stability,
• Increased revenue,
• Reduction of environmental footprint.

Biotechnologies are changing the world
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Lentikats Biotechnology

• Unique shape of the matrix and original
  immobilisation procedure enables to encapsulate
  free enzymes or microorganisms (bacteria, yeasts,
  fungi) inside a hydrogel porous carrier from
  polyvinyl alcohol (PVA). The advantages of PVA
  matrix are
• excellent physical and mechanical characteristics
  that result in a long-term mechanical stability
• hardly biodegradable
• no toxic effects
• Lentikats Biocatalysts enable easy separation of
  enzymes or microorganisms from the bulk media and
  therefore can be used repeatedly.

With biotechnologies to a better future
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Lentikats Biotechnology
Microencapsulation
vs.



traditional proven technology
new method based on well-proven principle
spherical shape limited diffusion to the core
lentil-like shape no diffusion limitations
micro size
mili size easier separation
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How does the Lentikats Biocatalyst work?

• A maximum surface in combination with the minimum
  volume of Lentikats Biocatalyst results in a
  substantial reduction of diffusion limitations
  associated with the transfer of substrate into
  the matrix and the product out of the matrix.

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Why polyvinylalcohol?

• It is an inexpensive material
• Encapsulation takes place under optimal,
  cell-sparing conditions
• It allows a production of hydrogel with excellent
  mechanical properties
• It does not have any side effects on fermentation
• It offers high capacity for the cell loading
• The process is stable and easy to control
• It is a non-toxic material

Biotechnologies are changing the world
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How is Lentikats Biocatalyst produced?
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Lentikats Biocatalysts

• Biocatalyst Types for Use in Lentikats
  Encapsulation
• Free Cells
• dead cells
• viable, non growing cells
• viable and growing cells
• Free or Immobilised Enzymes.
• Lentikats Biotechnology allows to immobilise any
  type of enzyme
• The size of Lentikats Biocatalyst pores varies
  depending on the size and diameter of the
  Biocatalyst due to
• PVA additive type (size of macromolecule)
• additive concentration
• biomass concentration

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Lentikats Biocatalysts Manufacturing Plant
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Laboratory Units for Encapsulation

• For low-capacity production of encapsulates
  (especially for research purposes), a laboratory
  device is available

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What are the benefits?
Higher reaction rates
Reduction of reaction tanks volume
Operational costs savings (electricity, heat)
Matrix containing high concentration of biomass
Higher yields (in comparison to conventional
technologies using free biomass)
Possibility of repeated usage
Operational costs savings
Enhanced resistance of modified and immobilised
biomass
Higher process stability
Easy separation of biomass from reaction media
Reduced costs for downstream processes
(separation)
Lower production of waste and by-products
Environmentally friendly disposal
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..and also

• Shortening of the reaction times
• Much higher yields in comparison to conventional
  technologies (up to 5 15 higher)
• Repetitive use of the Biocatalyst, easy
  separation from media (sieve separator)
• Higher productivity of the Biocatalyst
• Lower risk of contamination during the production
  process
• High stability and easy control of production
  process

Biotechnologies are changing the world
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Technological set-up
? - inlet of reaction media ? - filling of
Lentikats Biocatalyst ? - medium with Lentikats
Biocatalyst ? - stirrer ? - sieve separator ? -
outlet of separated media ? - outlet of Lentikats
Biocatalyst
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Lentikats and food industry

• We currently offer solutions for the following
  processes
• production of lactose free milk
• Lentikats Biocatalyst with ß-galactosidase
  activity
• production of invert sugar
• Lentikats Biocatalyst with invertase activity
• whey processing
• Lentikats Biocatalyst with ß-galactosidase
  activity for
• production of D-galactose from whey lactose
  (pharmaceutical quality)
• production of galacto-oligosaccharides from whey
  lactose
• production of special syrups
• Lentikats Biocatalyst with invertase activity for
  production of glucose-fructose syrups
• Lentikats Biocatalyst with immobilised
  glucoamylase for production of glucose from sugar
  and starch-based materials or glucose syrups from
  maltotriose or maltose syrup

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Lentikats and food industry

• Following solutions are currently under
  development and testing
• production of organic acids
• Lentikats Biocatalyst with immobilised bacteria
  Bacillus coagulans for production of lactic acid
  from lactose, glucose and sucrose
• Lentikats Biocatalyst with immobilised
  filamentous fungi Aspergillus niger for
  production of gluconic acid from sucrose and
  glucose
• Lentikats Biocatalyst with immobilised
  filamentous fungi Aspergillus terreus for
  production of itaconic acid from sucrose and
  glucose

Biotechnologies are changing the world
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Lentikats Biotechnology - examples

• LENTIKATS BIOTECHNOLOGY FOR PRODUCTION OF INVERT
  SUGAR
• enzyme immobilised saccharase,
  3-D-fruktofuranosidase for hydrolysis of sucrose
  into glucose and fructose.
• optimum temperature 3035C
• optimum pH 4,55,0
• conversion rate 100
• operational stability 600-1000 repeated batch
  conversions
• application - processing of molasses or sucrose
  solutions

source activity
Bakers yeast 600-1000 g sucrose/ kg LB/hr
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Lentikats Biotechnology - examples

• LENTIKATS BIOTECHNOLOGY FOR PRODUCTION OF LACTOSE
  FREE MILK

• enzyme immobilised laktase, ß-D-galactosid
  galactohydrolase for lactose hydrolysis.
• optimum temperature 3050C
• optimum pH 6,06,5
• conversion rate gt 90
• operational stability 120160 hours

source activity
Kluyveromycer lactis 100-300 g GLC/ kg LB/hr
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Investment and Investment Return Guarantees

• You can easily verify our great results by
  testing our Biocatalyst in your or other
  facilities with the material of your choice.
• Sample of Lentikats Biocatalyst free of charge
• Our company provides assistance and full
  technical support.
• Our company offers a long-term cooperation to
  companies, whose main concerns are reduced
  investment costs and maximal process
  profitability.

Biotechnologies change the world
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Investment and Investment Return Guarantees

• We guarantee the following
• output parameters
• operational stability of Lentikats Biocatalyst
  and its activity and productivity

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Our partners in the field of industrial
applications
Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India www.fermentabiotech.com
Protean, Pod Lesem 9, 373 16 Dobra Voda u Ceskych Budejovic, Czech Republic www.protean.cz
Pharmix s.r.o., Na Sadkach 2798, 767 01 Kromeriz, Czech Republic www.pharmix.cz
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Our partners in the field of research and
development
Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India www.fermentabiotech.com
Institute of Microbiology, Academy of Science of the Czech RepublicVídenská 1083, 142 20 Prague 4, Krc, Czech Republic www.biomed.cas.cz
STU Bratislava, Fakulta chemické a potravinárské technologie, Ústav biotechnologie a potravinárství, Radlinského 9, 81237 Bratislava, Slovakia www.stuba.sk
VŠCHT Praha, Ústav kvasné chemie a bioinženýrství, Technická 5, 166 28 Praguea 6 www.vscht.cz
University of Alberta, Edmonton Alberta, T6G 2J9 Canada www.ualberta.ca
Masaryk University, National Centre for Biomolecular Research, Kamenice 5, 625 00 Brno-Bohunice www.ncbr.chemi.muni.cz
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Thank you for attention
Company Headquarters LentiKats a.s. Evropska
423/178 160 00 Prague 6 Czech republic Tel.
420 224 362 461 Fax [phone]
E-mail info_at_lentikats.eu Production plant and
laboratories LentiKats a.s. Pod Vinicí 83 471 27
Stráž pod Ralskem Czech republic Tel. 420 487
851 393 Fax [phone] E-mail
info_at_lentikats.eu