Freimann Life Science Center (FLSC)

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Freimann Life Science Center Center for Zebrafish
Research
OVERVIEW
RESEARCH CONTRIBUTION HIGHLIGHT
LIST OF SERVICES

• Freimann Life Science Center (FLSC)
• State-of-the-art animal research facility
• 30,000 square feet, flexible use
• Fully accredited and registered
• Highly trained, professional staff
• Center for Zebrafish Research (CZR)
• Housed in approximately 3,000 square feet
• Consists of 8 double-sided racks and 38
  single-sided racks that allow for a flexible
  arrangement of tanks from 1.5 to 8 liters in size
  
• A separate room houses the zebrafish nursery
• Racks are maintained on multiple independent
  life-support systems to minimize a catastrophic
  event to the entire system
• Approximate total capacity 120,000 adult
  zebrafish (one of the largest systems in the
  Midwest)

• Freimann Life Science Center
• Histopathology preparation and histological
  interpretation of tissue samples.
• Technical and surgical services provided by
  skilled, experienced staff.
• Training of personnel for animal work.
• Rodent breeding colony management.
• Housing for rodents, rabbits, amphibians, fish,
  chickens, mini-pigs, non-human primates.
• Center for Zebrafish Research
• Generation of ENU-induced mutants and screening
  of mutant phenotypes.
• Introduction of morpholinos into embryos and some
  adult tissues to assay for loss-of-function
  phenotypes of specific target proteins.
• Microinjection of DNA constructs and subsequent
  analysis of transgenic lines.
• Training of individuals and groups in the above
  mentioned techniques and general zebrafish
  husbandry.

• Freimann Life Science Center
• Animal modeling for testing anti-cancer compounds
  and vaccines (collaborations with Penn State
  University School of Medicine, Indiana
  University, Medical College of Wisconsin,
  private industry).
• Histopathology support for cancer studies,
  toxicity evaluation, and biomedical engineering
  applications.
• Surgical services (e.g., experimental hernia
  repair tumor debulking wound repair studies
  biomaterial evaluation).
• Evaluation of imaging agents (collaborations with
  Goshen Center for Cancer Care and UND
  investigators).
• Research efforts over last three years have
  produced 30 published manuscripts, 14 meeting
  abstracts, and 5 patent applications.
• Intellectual property associated with novel
  vaccine adjuvant and cancer vaccine licensed to
  industry
• Center for Zebrafish Research
• Collaborations with groups from the University of
  Michigan, the Georgia Institute of Technology,
  and the University of North Carolina at Chapel
  Hill to generate a variety of transgenic
  zebrafish lines.

RESOURCES
FLSC State-of-the-art
individually Capability for housing
non-ventilated caging-standard human
primates for rodents Certified
technical staff available Lobund-Wistar rat
model of to perform or assist with surgical
metastasizing prostate cancer and other
technical procedures CZR
Fish are maintained on
Microinjection rigs are available an open
system that is to introduce DNA constructs
automated to control for the into embryos to
generate temperature, pH, and salinity.
transgenic lines (above) and The CZR currently
maintains morpholinos into embryos and three
wild-type stocks (AB, Wik, adults to determine
the and Tubingen), 34 mutant stocks,
loss-of-function phenotype and 95 transgenic
lines. of desired proteins.
QUALITY CONTROL AND ASSURANCES

• Freimann Life Science Center
• Routine rodent colony health monitoring
• Board-certified laboratory animal veterinarian
• Accredited by Association for Assessment and
  Accreditation of Laboratory Animal Care (AAALAC)
• PHS Assurance, USDA Registration

CONTACT INFORMATION

• Freimann Life Science Center
• Dr. Mark A. Suckow, DVM, Dipl. ACLAM,
• Director
• Tel. [phone] Email
  suckow.1_at_nd.edu
• Center for Zebrafish Research
• Dr. David R. Hyde, Ph.D.
• Howard J. McKenna C.S.C. Memorial Director
• Tel. [phone] Email dhyde_at_nd.edu