AstaREAL

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AstaREAL Astaxanthin Reducing Eye fatigue

• Clinical Summary

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Summary

• Eye fatigue suffers a global problem.
• Only 50 of sufferers respond to conventional
  measures e.g. ergonomics
• Astaxanthin alleviates eye fatigue by directly
  improving the ciliary body function that controls
  lens refraction.
• Astaxanthin reduces inflammation and improves
  retinal blood flow.

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Eye Fatigue Accommodation
Six independent facility (eight clinical trials)
demonstrated that eye fatigue and accommodation
were significantly improved after AstaREAL
supplementation.

1. Toyama Medical Pharmaceutical University
2. Fujita Health University School of Medicine
3. Hokkaido University School of Medicine x 2
4. Kajita Clinic

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1-1. Accommodation
A double masked study VDTVisual Display
Terminals 5 mg astaxanthin per day or placebo
during 4 weeks
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1-2. Eye Fatigue
A questionnaire before and after the
administration period.
54 reduction
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2. Accommodation
A double blind study 46 eye fatigue subjects
0 mg (placebo, n10) 2 mg (n10) 4 mg
(n13) 12 mg (n13) Astaxanthin per day or
placebo for 28 days
Dose Dependent Effect!!
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3-1. Dose Finding Study-1
Sub-objective Accommodation Power
A double blind study 30 eye fatigue subjects
0 mg (placebo, n10) 6 mg (n10) 12 mg
(n10) Astaxanthin per day or placebo for 4
weeks Singificantly increased by 12mg
supplementation at week 4 compared to start
plt0.05
Objective Accommodation Power (D)
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3-1. Dose Finding Study-2
Positive Accommodation Time
- 6mg supplementation Significantly shortened
at week 4 compared to start - 12mg
supplementation Also significantly shortened at
week 4 compared to start plt0.05


Positive Accommodation Time (D/s)
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3-1. Dose Finding Study-3
Negative Accommodation Time
Plt0.05
- 6mg supplementation Singificantly shortenend
at week 4 compared to start placbo
Optimal Dose 6mg/day
Negative Accommodation Time (D/s)
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3-2. Efficacy-Identification Study-1
Sub-objective Accommodation Power
A double blind study 40 eye fatigue subjects
0 mg (placebo, n20) 6 mg (n20) Astaxanthin
per day or placebo for 4 weeks Sigificantly
higher at week 2 4 compared to start placebo
Sub-objective Accommodation Power Rate of Change
()
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3-2. Efficacy-Identification Study-2
Positive Negative Accommodation Time
Positive Accommodation Time Rate of Change ()
Negative Accommodation Time Rate of Change ()
Sigificantly higher at week 2 4 compared to
start placebo
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4. Accommodative recovery
HFC (High Frequency Component in Accommodative
Mirco-Fluctuation of ciliary body)
10 healthy subjects (Male 5, Female 5) HFC
difference was calculated between HFC at VDT
(visual display terminal) working for 30min and
HFC at 20min rest after VDT. The difference was
measured before and after 6 mg astaxanthin/day
for 2 weeks. Increase of HFC after the rest was
singificantly restrained by astaxanthin
supplementation.
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Visual Acuity for Sports Performance
Sawaki,K. et al. 2002, Journal of Clinical
Therapeutics Medicines, 18(9) 73-88.
- Double blind placebo controlled studies on
male handball atheletes - 6 mg (n9)
astaxanthin vs placebo (n9) for 4 weeks -
Improving of depth perception critical flicker
fusion - improved ciliary muscle function for
visual acuity
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Visual Acuity-1-Depth Perception-




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Visual Acuity-2-Critical Flicker Fusion -
Photoreceptors processed light better in
astaxanthin group.
Before Ex.
After Ex.
plt0.05
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Why Astaxanthin Reduces Eye Fatigue?
1. Retinal Blood Flow
A double blind study 36 healthy subjects 0 mg
(placebo, n18) 6 mg (n18) Astaxanthin per day
or placebo for 4 weeks Singificantly increased
in the astaxanthin supplemented group at week 4
in both eyes
Nagaki,Y. et al. 2005, J. Clinical Therapeutics
Medicines 21(5)537(73)-542(78).
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Why Astaxanthin Reduces Eye Fatigue?
2. Anti-Inflammation
Measured in the aqueous humor The aqueous humor
was collected 24hr after LPS treatment.
Pred.10mg/kg Prednisolone by iv as a positive
control
Astaxanthin suppressed the development of
endotoxin-induced uveitis in rats. The
anti-inflammatory effect of 100 mg/kg Ax was as
strong as that of 10 mg/kg prednisolone.
Ohgami,K. et al. 2003, Invest Ophthalmol Vis Sci.
44(6)2694-2701. New publication 2006 also
available.
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Eye Health -rat model-
Measured in the aqueous humor The aqueous humor
was collected 24hr after LPS treatment.
Pred.10mg/kg Prednisolone by iv as a positive
control
Astaxanthin suppressed the development of
endotoxin-induced uveitis in rats
K. Ohgami et al., Invest Ophthalmol Vis Sci.
44(6), 2694-2701 (2003).
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Action Mechanism of Anti-inflammation by
Astaxanthin
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PGE2
COX-2
DNA
iNOS
m-RNA
NO
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Astaxanthin suppresses NF-kB activation
Normal iris-ciliary body
LPS administration
LPS Astaxanthin
? NF-kB positive cells
plt0.01
NF-kB p65 were immunostained with
antibodies Green normal nuclei Red NF-kB p65
Experimental Eye Reaseach, in press
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Conclusion

• Astaxanthin
• reduced ciliary muscle strain during eye
  fatigue
• improved ciliary muscle function for visual
  acuity
• by increasing the
• retinal blood flow
• by inhibiting the
• inflammation in
• the eyes

US JP Patent pending