PowerPoint slides to accompany

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LESSON 4
PowerPoint slides to accompany Using
Bioinformatics Genetic Testing
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LABORATORY TECHNICIANZANE KRAFT, MS
Place of Employment Seattle Biomedical Research
Institute Type of Work HIV Vaccine Research
We do a lot of DNA alignments of HIV Envelope
sequences. Another fun thing that we now do more
of is computer modeling of HIV Envelope proteins
with different mutations.
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Inside the Gene Machine How Information from DNA
is Acquired and Used for Genetic Testing
Genetic Counselors work with patients to help
them decide whether to have a genetic test, and
help them understand the results of the test.
Patient Sample Blood or Saliva
Lab Technicians work with patient samples in the
lab, purifying and sequencing the DNA.
Extract DNA from Cells
Computational Biologists create computer programs
to help biologists analyze data.
Sequence DNA
Biomedical Researchers perform experiments with
patient samples to find different variations of
genes that might cause disease.
Reference TTCACCAACATGCCCACA F T
N M P T Patient TTCACCAACAGGCCCACA
F T N R P T
Compare Patient DNA Sequence to Reference
Sequence
Search Database to Determine whether Patient
Mutation is Associated with Disease
Bioengineers help create tools and treatments for
patients with various diseases. Medical Doctors
and Veterinarians use the knowledge gained from
genetic testing to care for their patients.
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The BRCA1 Gene
ATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGC
TATGCAGAAAATCTTAGAGTGTCCCATCTGTCTGGAGTTGATCAAGGAAC
CTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAA
CTTCTCAACCAGAAGAAAGGGCCTTCACAGTGTCCTTTATGTAAGAATGA
TATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTG
AAGAGCTATTGAAAATCATTTGTGCTTTTCAGCTTGACACAGGTTTGGAG
TATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACA
TCTAAAAGATGAAGTTTCTATCATCCAAAGTATGGGCTACAGAAACCGTG
CCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACC
AGTCTCAGTGTCCAACTCTCTAACCTTGGAACTGTGAGAACTCTGAGGAC
AAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGAT
CTGATTCTTCTGAAGATACCGTTAATAAGGCAACTTATTGCAGTGTGGGA
GATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAG
TTTGGATTCTGCAAAAAAGGCTGCTTGTGAATTTTCTGAGACGGATGTAA
CAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAG
AAGCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAGGGTAGTTCTGTTTC
AAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTAC
AGCATGAGAACAGCAGTTTATTACTCACTAAAGACAGAATGAATGTAGAA
AAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCA
ACATAACAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTC
CCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGA
AAAGAATGGAATAAGCAGAAACTGCCATGCTCAGAGAATCCTAGAGATAC
TGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATG
AGTGGTTTTCCAGAAGTGATGAACTGTTAGGTTCTGATGACTCACATGAT
GGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAA
TGAGGTAGATGAATATTCTGGTTCTTCAGAGAAAATAGACTTACTGGCCA
GTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAA
TCAGTAGAGAGTAATATTGAAGACAAAATATTTGGGAAAACCTATCGGAA
GAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAG
GAGCATTTGTTACTGAGCCACAGATAATACAAGAGCGTCCCCTCACAAAT
AAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTT
TATCAAGAAAGCAGATTTGGCAGTTCAAAAGACTCCTGAAATGATAAATC
AGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAAT
AGTGGTCATGAGAATAAAACAAAAGGTGATTCTATTCAGAATGAGAAAAA
TCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAG
CTGAACCTATAAGCAGCAGTATAAGCAATATGGAACTCGAATTAAATATC
CACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTAC
CAGGCATATTCATGCGCTTGAACTAGTAGTCAGTAGAAATCTAAGCCCAC
CTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATA
AAGAAAAAAAAGTACAACCAAATGCCAGTCAGGCACAGCAGAAACCTACA
ACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGC
CAAATGAACAGACAAGTAAAAGACATGACAGCGATACTTTCCCAGAGCTG
AAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGA
ACTTAAAGAATTTGTCAATCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGA
AACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTC
ATGTTAAGTGGAGAAAGGGTTTTGCAAACTGAAAGATCTGTAGAGAGTAG
CAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCT
CGTTACTGGAAGTTAGCACTCTAGGGAAGGCAAAAACAGAACCAAATAAA
TGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGG
TTGTTCCAAAGATAATAGAAATGACACAGAAGGCTTTAAGTATCCATTGG
GACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGT
GAACTTGATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAAGCGCCA
GTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAA
CATTCTCTGCCCACTCTGGGTCCTTAAAGAAACAAAGTCCAAAAGTCACT
TTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATAT
CAAGCCTGTACAGACAGTTAATATCACTGCAGGCTTTCCTGTGGTTGGTC
AGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCT
AGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAACTGGACTCATTAC
TCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTT
TTCCCATCAAGTCATTTGTTAAAACTAAATGTAAGAAAAATCTGCTAGAG
GAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGA
GAACATTCCAAGTACAGTGAGCACAATTAGCCGTAATAACATTAGAGAAA
ATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGT
ACTAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAGTGATGAAAA
CATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGC
TTAGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTTCCTGGA
AGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGT
TCAGACTGTTAATACAGATTTCTCTCCATATCTGATTTCAGATAACTTAG
AACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCT
GATGACCTGTTAGATGATGGTGAAATAAAGGAAGATACTAGTTTTGCTGA
AAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAG
GAGAGCTTAGCAGGAGTCCTAGCCCTTTCACCCATACACATTTGGCTCAG
GGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATC
TAGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAG
TAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAG
TGTCTGTCTAAGAACACAGAGGAGAATTTATTATCATTGAAGAATAGCTT
AAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATC
ACCTTAGTGAGGAAACAAAATGTTCTGCTAGCTTGTTTTCTTCACAGTGC
AGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTT
GATTGGTTCTTCCAAACAAATGAGGCATCAGTCTGAAAGCCAGGGAGTTG
GTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGC
TTGGAAGAAAATAATCAAGAAGAGCAAAGCATGGATTCAAACTTAGGTGA
AGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAG
GGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGATACCATG
CAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGT
GTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCATCATAA
GTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCA
GAAAAAGCAGTATTAACTTCACAGAAAAGTAGTGAATACCCTATAAGCCA
GAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTT
CTACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGTCATCCCCTTCTAAA
TGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCT
TCAGAATAGAAACTACCCATCTCAAGAGGAGCTCATTAAGGTTGTTGATG
TGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACA
TCTTACTTGCCAAGGCAAGATCTAGAGGGAACCCCTTACCTGGAATCTGG
AATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAG
CCCCAGAGTCAGCTCGTGTTGGCAACATACCATCTTCAACCTCTGCATTG
AAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGC
TCATACTACTGATACTGCTGGGTATAATGCAATGGAAGAAAGTGTGAGCA
GGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATG
TCCATGGTGGTGTCTGGCCTGACCCCAGAAGAATTTATGCTCGTGTACAA
GTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGA
CTACTCATGTTGTTATGAAAACAGATGCTGAGTTTGTGTGTGAACGGACA
CTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTT
CTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATT
TTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAG
CGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTG
TTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGG
TACAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTT
GGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGA
GGACAATGGCTTCCATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGA
CCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTG
GACACCTACCTGATACCCCAGATCCCCCACAGCCACTACTGA
http//research.nhgri.nih.gov/projects/bic/Member/
brca1_mutation_database.shtml
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LABORATORY TECHNICIANZANE KRAFT, MS
Place of Employment Seattle Biomedical Research
Institute Type of Work HIV Vaccine Research
We do a lot of DNA alignments of HIV Envelope
sequences. Another fun thing that we now do more
of is computer modeling of HIV Envelope proteins
with different mutations.
6
CAREERS IN SPOTLIGHTLABORATORY TECHNICIAN
What do they do? Laboratory technicians,
sometimes called lab techs or research
technicians, do much of the day-to-day work in
the lab, including purifying and sequencing DNA,
caring for laboratory machinery, and analyzing
samples. What kind of training is involved? Some
laboratory technicians have a two year
Associates degree or a four year Bachelors
degree. Some, like Zane Kraft, also have a
Masters degree (additional two year
training). What is a typical salary for a
Laboratory Technician? Ranges with experience
from 30,000/year (14/hour) up to 60,000/year
(29/hour) or more.
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