Compliance

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Compliance
Its easier than you think!!
Office of the IACUC
Mike Andrews IACUC Training and Compliance
Coordinator
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We as a research community are charged by
the public with the responsibility to assure
animal welfare in exchange for the privilege of
utilizing animals in the advancement of human
welfare.
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Just a few basics
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IACUC Committee
Vet Review
IACUC Administrator
DMR
Approved for 3 years
2 years, 9 months Time to renew!
Administrative DMR Committee
Amendments?
PAM every 6 months
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Definitions
IACUC Institutional Animal Care and Use
Committee OAR Office of Animal Resources PHS
NIH Public Health Services National
Institutes of Health OLAW Office of Laboratory
Animal Welfare AAALAC Association for
Assessment and Accreditation of Laboratory
Animal Care International The Guide
The Guide to the Care and Use of Laboratory
Animals USDA United States Department of
Agriculture APHIS Animal and Plant Health
Inspection Services AWA Animal Welfare
Act Compliance Following all regulations set
forth in the PHS Policy on Humane Care and Use
of Laboratory Animals, the Guide and
AWA Reportable Event Departure from guidelines
that is reported to Grantors
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Regulatory Oversight of Animals Used in Research
USDA (United States Department Of Agriculture)
PHS (Public Health Services)
AAALAC, International (Association for the
Assessment and Accreditation of Laboratory
Animal Care International)
NIH (National Institute Of Health)
OLAW (Office of Laboratory Animal Welfare)
APHIS (Animal and Health Plant Inspection
Services)
AWA (Animal Welfare Act) The Guide (The Guide
for the Care and Use of Laboratory Animals)
UNIVERSITY OF IOWA IACUC (Institutional Animal
Care Use Committee) AP or Protocol (Animal
Protocol) Investigator
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USDA has announced this is the
AGE OF ENFORCEMENT
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The new Guide is out
The IACUC and PAM are here to guide you through
the new Guide Educate Resource Connect
http//nexus.od.nih.gov/nexus/nexus.aspx?ID473Mo
nth06Year2010
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The IACUC and PAM are not the cops!!!
Information on Animal Protocols remains
up-to-date and in compliance. Foster a teamwork
environment between the laboratories and
IACUC. Allow for an exchange of information and
education. Create consistency in policies which
allows consistency in research performed at the
University. Our purpose is to allow your
research to proceed without interruption or
jeopardy!
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Some common Compliance issues
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Uncovered cages in public areas

• All cages and transport vehicles, regardless of
  whether they contain animals or not, must be
  covered when outside of Animal Resources
  facility.
• Animals\caging must not be visible.
• Carry no more than 2 mouse/rat cages.
• Must use cart for more than 2 mouse/rat
• cages.

Material covering the cage must be oxygen
permeable. A plastic garbage is not an
acceptable method!!
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If your mouse is looking back at you, he is NOT
covered!
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Animals being housed in laboratories without
prior approval
Animals cannot be kept outside of the animal
husbandry facilities for more than 12 hours (USDA
species) or 24 hours (rodents) without IACUC
approval.
This is a federal requirement!!! This request
requires the convening of the full IACUC before
approval can be granted!!
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Not cleaning up animal waste
You are responsible for cleaning up after your
animals! University custodians are not allowed
to handle animal waste.
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Proper Use of CO2 in Euthanasia Procedures

• Animals should remain in original housing cage.
• DO NOT COMBINE animals from any other cage.

• Filter top must remain on cage.
• Gently insert tube through water hole flap.

DO NOT TOUCH!

• Turn on CO2 gas to Level 15.
• Maintain gas flow until
• breathing stops, AND
• Continue gas flow for 1
• additional minute.

• Turn off CO2 gas.

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• Remove tube.
• Leave animals in cage with CO2 for at least
• 5 more minutes.

Flap must remain closed
Filter top must remain in place
Flap must remain closed

• Perform confirmation of death as described
• in the approved Animal Protocol.

• After confirmation of death has been performed,
  place animalsin plastic bag.
• Tie a knot in the bag.
• Place bag into a red container in the cooler.

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Confirmation of death must be performed on every
animal

• It is required that a confirmation of death be
  performed
• on every animal being euthanized. The method of
  confirmation must be identified in the approved
  Animal Protocol.
• Examples
• Animals under 500 gm
• Cervical dislocation
• Decapitation
• 15 minutes in a CO2 charged chamber
  post-respiratory arrest
• Thoracotomy
• Vital tissue harvest (must include brain, heart
  and/or lungs)
• Animals over 500 gm
• Bilateral thoracotomy
• Vital tissue harvest (must include brain, heart
  and/or lungs)

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Misuse of animal access Cameras/cell phones

• No photos are allowed in the animal housing
  facility with a cell phone or any other camera,
  except with written authorization from the Office
  of Animal Resources.

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Misuse of animal access Entering facilities

• Do not allow people you do not know personally
  into the facilities.
• Do not admit other people into the animal
  facility or animal rooms.
• You are authorized only in animal rooms where
  your animals are housed.

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The Definition of Surgery Disruption of any
integumentary surface of a living animal by any
means other than a hypodermic needle, biopsy
needle, ear punch, or tail snip is considered
surgery. 
All persons performing survival surgeries must
be observed performing one surgery

• Surgical reports must be maintained!
• Surgical records are a record of what
• actually happened, not a reiteration
• of what is stated in the Animal Protocol
• Date
• Animal Protocol number
• Animal ID
• Procedure
• Anesthetic agents
• Time of induction
• Time of recovery
• Date(s) and time(s) of analgesia
• Post-op monitoring
• Date when sutures are removed
• or are no longer present

If its not written down, it didnt happen!!
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Where are your Animal protocols???
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Performing procedures on The wrong animal
or Animal Protocol (AP)
Before performing any procedures, double-check
the AP number on the animal cage to the approved
AP to verify that the procedure is approved under
that AP number.
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Performing unapproved procedures
Before performing any procedures, double-check
that the procedure to be performed is approved
under that AP number. A description of the
procedure must be approved on an AP before it can
be performed.
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Unapproved Personnel

• Before performing any procedures, double-check
  that the person who is about to perform a
  procedure is listed on that AP number.
• It is required that each person working with
  animals must be listed on each AP.
• You ARE NOT approved if
• You are listed on one approved AP, but are
  handling the animals approved under another
• Your PI is listed on an approved AP, but you are
  not
• An amendment has been sent in, but not yet
  approved

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Incorrectly Transferring Animals Between PIs
Animals transferred to a different PI and AP
cannot be used until transfer has been
approved!!! Signatures from both labs must be
obtained.
Originating lab
Receiving lab
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How to Transfer Animals Between PI/Labs

• Originating PI/lab tech labels each cage to be
  transferred with a Purple Transfer Card (obtained
  from the Area Supervisor).
• Originating PI/lab tech fills out the top section
  of a yellow mouse slip for Transfer Mice.
  Originating lab must sign transfer slip.
• Receiving PI/lab tech must fill out the bottom
  section of the transfer slip. Receiving PI/lab
  must sign the transfer slip.
• After both signatures have been obtained
• Give to area Supervisor, OR
• Give to area Caretaker, OR
• Leave slip on one of the labeled cages to be
  transferred.

Receiving PI/lab CANNOT touch, use or move the
transferred animals until the Receiving PI/lab
has received an email from OAR stating that the
transfer has been approved.
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How to Transfer Animals Between Rooms

1. PI/lab fills out yellow mouse slip for Transfer
   Mice.
2. If OAR is moving the animals
3. Label cages with a Purple Transfer Card (obtained
   from the area Supervisor) to be moved by OAR
4. Leave slip on one of the labeled cages to be
   moved, OR
5. If the PI/lab is moving the animals
6. Obtain a signature from the area
   Supervisor/Caretaker (to ensure the building/room
   change is suitable and available)
7. PI/lab can then move the cages
8. This option is only available when a car or van
   is not required for the move

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To summarize
KNOW YOUR Animal Protocol!!!
If you are not sure, ASK!!!
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Questions
Concerns?
Comments?