Riboswitch Functions

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Riboswitch Functions

• Direct (protein-free) binding of metabolite to
  RNA
• Metabolite-dependent regulation of genes

These two activities are accomplished by two
functionally separate domains on the RNA
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3
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2
1
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Courtesy Ron Breaker Lab (Yale University)
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Riboswitch Mechanisms
They also affect RNA integrity, and perhaps
splicing and stability.
Ligand binding leads to mRNA cleavage by a new
natural ribozyme.
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Ligand binding could control splicing in
eukayotes.
Possibly, ligand binding to the 3 untranslated
region could affect mRNA stability.
Courtesy Ron Breaker Lab (Yale University)
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Riboswitch Ligands
There are 8 confirmed riboswitches with unique
metabolite ligands.
Many more conserved RNA motifs are currently
under investigation.
Courtesy Ron Breaker Lab (Yale University)
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Courtesy Ron Breaker Lab (Yale University)
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Manufacturing of Affy GeneChip
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Affymetrix E. coli GeneChip Image