Enzyme Bead Simulation

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Enzyme Bead Simulation
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Enzyme -

• Organic catalyst
• speeds up reactions
• proteins
• lower activation energy Ea
• can be reused over and over

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COENZYME -

• 1/2 enzyme
• 1/2 vitamin
• enhance the effect of an enzyme

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COOPERATIVE GROUP ROLES

• Recorder - writes down data
• Grabber - gets the beads
• Polymer maker - adds the beads to the growing
  chain

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TRIAL 1 - baseline

• Pick up your specific bead color and run it back
  to your desk to be added to the growing polymer
  chain
• timing is done every 10 seconds

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TRIAL 2 - Coenzyme

• Same as the control but the grabber can throw the
  bead back to the polymer maker

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TRIAL 3 - Denaturation

• Same as the baseline but you must tape together
  your fingers
• that means NO THROWING back to the polymer
  maker!!!!

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TRIAL 4 Competitive Inhibition

• same as the baseline but you must bounce the ping
  pong ball catch it then get your bead

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Table 1 Group Data
Time (sec) Trial 1 Baseline Trial 2 Coenzyme Trial 3 Denaturation Trial 4 Comp. Inh.
0-10 3 6 2 2
10-20 6 12 3 5
20-30 9 18 5 7
30-40 12 24 6 11
40-50 15 30 8 13
50-60 18 36 10 16
60-70 21 42 11 18
70-80 24 48 13 21
80-90 27 54 15 23
90-100 31 59 16 25
100-110 33 64 18 27
110-120 35 69 20 29
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Processing the Data

• Set up a graph of all of the data with the number
  of beads on the Y axis, Time (10 sec increments)
  on the X axis, a key for all 4 trials and the
  title being
• Figure 1 Effects on Enzyme Action

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Analysis Questions

• Refer to the packet for the questions.
• To complete the lab
• Fill in data
• Make the graph
• Answer the questions ?