An Introduction to Ultraviolet/visible Molecular Absorption Spectrometry

1
An Introduction to Ultraviolet/visible Molecular
Absorption Spectrometry
2

• 1 Measurement of transmittance and absorbance
• 2 Beers law
• 3 The effects of instrumental noise on
  spectrophotometric analysis
• 4 Instrumentation

3
1 Measurement of Transmittance and Absorbance
4
2 Beers law

• 2-1 application of beers law to mixtrures
• 2-2 limitation to beers law

5
3 The Effects of Instrumental Noise on
spectrophotometric Analysis

• 3-1 instrumental noise as a function of
  transmittance
• 3-2 sources of instrumental noise
• 3-3 effect of slit width on absorbance
  measurements
• 3-4 effect of scattered radiation at wavelength
  extremes of an instrument

6
4 Instrumentation

• 4-1 instrument components
• 4-2 types of instruments
• 4-3 some typical instruments

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Applications of Ultraviolet/visible Molecular
Absorption Spectrometry
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• 1 the magnitude of molar absorptivities
• 2 absorbing species
• 3 application of absorption measurement to
  qualitative analysis
• 4 quantitative analysis by absorption
  measurements
• 5 Photometric titrations
• 6 Photoacoustic spectroscopy

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1 the magnitude of molar absorptivities
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2 Absorbing Species

• 2-1 absorbing species containing p,s,and n
  electrons
• 2-2 absorption involving d and f electrons
• 2-3 charge-transfer absorption

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3 application of absorption measurement to
qualitative analysis

• 3-1 methods of plotting spectral data
• 3-2 solvents
• 3-3 detection of functional groups

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4 Quantitative Analysis by Absorption Measurements

• 4-1 scope
• 4-2 procedural details
• 4-3 derivative and dual-wavelength
  spectrophotometr

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5 Photometric titrations

• 5-1 titration curve
• 5-2 instrumentation
• 5-3 application of photometric titrations

14
6 Photoacoustic Spectroscopy

• 6-1 the photoacoustic effect
• 6-2 photoacoustic spectra
• 6-3 instruments
• 6-4 applications