Assay for an Antibiotic Resistance Enzyme

1
Assay for an Antibiotic Resistance Enzyme

• Carolina Kit

2
Timeline

• TuesdayLecture,Review concepts of procedure
• ThursdayPart 1
• Part 2 (volunteer to serve as demonstrator4
  hours lab service)
• Monday--handouts due

3
Prep. For labAssay for Anti-biotic resistance

• 2 days prior (Tuesday)
• --label tubes control (8--15mL tubes)
• pAMP (8--15 mL tubes)
• penicillin-G (8--15 mL tubes)
• --streak fresh plate of MM294 and MM294/pAMP (24
  hours at 37 incubator)
• 1 day prior (Wednesday)
• --prepare cultures (1/group)see teacher guide
  (24 hours at 37 incubator)
• --penicillin-G solution (store at -20 until use)
• --aliquot (8 groups)
• MM294 5ml overnight culture, label control
• MM294/pAMP 5ml overnight culture, label pAMP
• 5ml of 4mg/mL penicillin-G
• On lab day 1 (Thursday)
• --test the reagents with phenol red
• Lab day 2 (Monday)
• --demonstrator of spectrometer

4
Write-up

• Complete all aspects of the handout (50 points)
• Staple these together. Drawings should be neat!