Pathogens must defend themselves against numerous factors

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Pathogens must defend themselves against numerous
factors
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Annual Reviews
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Annual Reviews
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Annual Reviews
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Annual Reviews
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http//plantpath.caes.uga.edu/personnel/faculty/Hu
ssey.html
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Secreted proteins with ortholog in M. hapla genome
Accession Number
Description

AF013289 Meloidogyne incognita secreted protein
MSP-1 AF049139 Meloidogyne incognita cellulose
binding protein precursor cbp-1 AF095949
Meloidogyne javanica chorismate mutase (NC30)
AF100549 Meloidogyne incognita
beta-1,4-endoglucanase (eng-1) AF323086
Meloidogyne incognita beta-1,4-endoglucanase
(eng-2) AY422836 Meloidogyne incognita
cellulase (eng3) AY422837 Meloidogyne
incognita cellulase (eng4) AF127915 Globodera
rostochiensis pectate lyase 1 precursor AF224342
Meloidogyne incognita xylanase (xyl-1) AF273735
Heterodera glycines hypothetical esophageal
gland cell secretory protein 8 (hsp8) AF374388
Heterodera glycines vap-1 gene AF402308
Meloidogyne incognita myosin regulatory light
chain AF402309 Meloidogyne incognita 14-3-3
product AF402771 Meloidogyne incognita
calreticulin AF468679 Heterodera glycines
chitinase AF469059 Heterodera glycines annexin
4C10 AF469060 Heterodera glycines ubiquitin
extension protein AF473831 Heterodera glycines
ubiquitin extension protein AF500024
Heterodera glycines putative gland protein G8H07
AF502391 Heterodera glycines putative gland
protein G10A06 AF520566 Heterodera glycines
pectate lyase 2 AF527788 Meloidogyne incognita
pectate lyase AF531160 Meloidogyne incognita
putative esophageal gland cell secretory protein
1 (msp1) AF531161 Meloidogyne incognita
putative esophageal gland cell secretory protein
2 (msp2) AF531162 Meloidogyne incognita
putative esophageal gland cell secretory protein
3 (msp3) AF531163 Meloidogyne incognita
putative esophageal gland cell secretory protein
4 (msp4) AF531164 Meloidogyne incognita
putative esophageal gland cell secretory protein
5 (msp5) AF531165 Meloidogyne incognita
putative esophageal gland cell secretory protein
6 (msp6) AF531166 Meloidogyne incognita
putative esophageal gland cell secretory protein
7 (msp7) AF531167 Meloidogyne incognita
putative esophageal gland cell secretory protein
11(msp11) AF531168 Meloidogyne incognita
putative esophageal gland cell secretory protein
8 (msp8) AF531169 Meloidogyne incognita
putative esophageal gland cell secretory protein
9 (msp9) AF531170 Meloidogyne incognita
putative esophageal gland cell secretory protein
10(msp10) AY134431 Meloidogyne incognita
putative esophageal gland cell secretory protein
12 (msp12) AY134432 Meloidogyne incognita
putative esophageal gland cell secretory protein
13 (msp13)
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Secreted proteins with ortholog in M. hapla
genome ..continued
Accession Number
Description
AJ243736 Globodera rostochiensis mRNA for
peroxiredoxin (tpx gene) AJ251757 Globodera
rostochiensis mRNA for for hypothetical protein
(clone A18) AJ270995 Globodera rostochiensis
mRNA for putative amphid protein (ams1
gene) AJ300178 Globodera pallida mRNA for
annexin 2 (nex-2 gene) AJ311902 Globodera
rostochiensis mRNA for EXPB2 protein AJ457834
Globodera pallida mRNA for putative chorismate
mutase (cm-1 gene) AJ493677 Globodera
rostochiensis mRNA for secreted glutathione
peroxidase (gpx1 gene) AJ557572 Meloidogyne
incognita mRNA for putative cathepsin L protease
(cpl-1 gene) AY026357 Heterodera glycines
pectinase precursor AY033601 Heterodera
glycines venom allergen-like protein (vap-2)
AY098646 Meloidogyne incognita
polygalacturonase AY134433 Meloidogyne
incognita putative esophageal gland cell
secretory protein 14 (msp14) AY134434
Meloidogyne incognita putative esophageal gland
cell secretory protein 15 (msp15) AY134436
Meloidogyne incognita putative esophageal gland
cell secretory protein 17 (msp17) AY134437
Meloidogyne incognita putative esophageal gland
cell secretory protein 18 (msp18) AY134438
Meloidogyne incognita putative esophageal gland
cell secretory protein 19 (msp19) AY134439
Meloidogyne incognita putative esophageal gland
cell secretory protein 20 (msp20) AY134440
Meloidogyne incognita putative esophageal gland
cell secretory protein 21 (msp21) AY134442
Meloidogyne incognita putative esophageal gland
cell secretory protein 23 (msp23) AY134443
Meloidogyne incognita putative esophageal gland
cell secretory protein 24 (msp24) AY134444
Meloidogyne incognita putative esophageal gland
cell secretory protein 25 (msp25) AY135362
Meloidogyne incognita putative esophageal gland
cell secretory protein 26 (msp26) AY135363
Meloidogyne incognita putative esophageal gland
cell secretory protein 27 (msp27) AY135364
Meloidogyne incognita putative esophageal gland
cell secretory protein 28 (msp28) AY135365
Meloidogyne incognita putative esophageal gland
cell secretory protein 29 (msp29) AY142116
Meloidogyne incognita putative esophageal gland
cell secretory protein 32 (msp32) AY142117
Meloidogyne incognita putative esophageal gland
cell secretory protein 34 (msp34) AY142118
Meloidogyne incognita putative esophageal gland
cell secretory protein 33 (msp33) AY142119
Meloidogyne incognita putative esophageal gland
cell secretory protein 35 (msp35) AY142121
Meloidogyne incognita putative esophageal gland
cell secretory protein 31 (msp31) AY160225
Heterodera glycines chorismate mutase (cm-1)
AY286305 Heterodera schachtii ubiquitin
extension protein (Ubi1) AY288520 Heterodera
schachtii ubiquitin extension protein 2 Y09293
Globodera pallida mRNA for putative SEC-2
protein DQ841123 Meloidogyne hapla
parasitism protein 16D10
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Secreted proteins with No ortholog in M. hapla
genome ..continued
AY134441 Meloidogyne incognita putative
esophageal gland cell secretory protein 22
(msp22) AY142120 Meloidogyne incognita putative
esophageal gland cell secretory protein 30
(msp30) AY134435 Meloidogyne incognita putative
esophageal gland cell secretory protein 16
(msp16) AF273728 Heterodera glycines
hypothetical esophageal gland cell secretory
protein 1 (hsp1) AF469058 Heterodera
glycines cellulose binding protein AJ251758
Globodera rostochiensis partial mRNA for
hypothetical protein (clone A41) AJ271910
Globodera rostochiensis mRNA for putative
hypodermis secreted protein (sxp1 gene) AF344862
Heterodera glycines putative salivary
proline-rich glycoprotein precursor Hgg-15
homolog from M. incognita (Accession number
AF049139) identified in M. hapla 16D10 (CLE)
positively identified (Accession number DQ841123)
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Secreted proteins with ortholog in C. elegans
Accession Number
Description
AJ557572 Meloidogyne incognita mRNA for putative
cathepsin L protease (cpl-1 gene) AF402309
Meloidogyne incognita 14-3-3 product AF402308
Meloidogyne incognita myosin regulatory light
chain AF013289 Meloidogyne incognita secreted
protein MSP-1 (msp-1) AF531162 Meloidogyne
incognita putative esophageal gland cell
secretory protein 3 (msp3) AF531167 Meloidogyne
incognita putative esophageal gland cell
secretory protein 11 (msp11) AF531170
Meloidogyne incognita putative esophageal gland
cell secretory protein 10 (msp10) AY135363
Meloidogyne incognita putative esophageal gland
cell secretory protein 27 (msp27) AY135364
Meloidogyne incognita putative esophageal gland
cell secretory protein 28 (msp28) AY142116
Meloidogyne incognita putative esophageal gland
cell secretory protein 32 (msp32) AY142121
Meloidogyne incognita putative esophageal gland
cell secretory protein 31 (msp31) Y09293
Globodera pallida mRNA for putative SEC-2
protein AJ493677 Globodera rostochiensis mRNA
for secreted glutathione peroxidase (gpx1
gene) AJ243736 Globodera rostochiensis mRNA for
peroxiredoxin (tpx gene) AF374388 Heterodera
glycines vap-1 gene AF402771 Meloidogyne
incognita calreticulin AY286305 Heterodera
schachtii ubiquitin extension protein (Ubi1)
AY288520 Heterodera schachtii ubiquitin
extension protein 2 AF473831 Heterodera
glycines ubiquitin extension protein AF469060
Heterodera glycines ubiquitin extension protein
AF468679 Heterodera glycines chitinase
AF500024 Heterodera glycines putative gland
protein G8H07 AF502391 Heterodera glycines
putative gland protein G10A06 AF344862
Heterodera glycines putative salivary
proline-rich glycoprotein precursor Hgg-15
AF273735 Heterodera glycines hypothetical
esophageal gland cell secretory protein 8 (hsp8)
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Host Selective Toxins
Only active on plant host of pathogen Over 20
known examples Structurally diverse - range from
low molecular weight metabolites to
proteins All known HSTs produced by fungi
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HSTs are required for pathogenicity
No toxin no disease Host sensitivity
conditioned by single gene Toxin damage to host
facilitates pathogenesis HSTs are Agents of
Compatibility
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Avirulence determinants
products of avr genes, confer incompatibility
to the pathogen in the presence of the
resistance gene no avr OR no R
disease incompatibility HR, therefore avr
products are Agents of Incompatibility
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The central conundrum both susceptible
interactions mediated by HSTs and resistance
responses mediated avr products result in HOST
CELL DEATH! -what distinguishes host response?
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The HR cell death interaction feedback
mechanism between reactive oxygen intermediates
and SA superoxide dismutase may play a key
role balance between NO and superoxide superox
ide is key regulator rapidly induced by SA
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The hypersensitive response is induced by
virulence gene products eflux of potassium, and
hydroxide influx of calcium and hydrogen
ions oxidative burst ROI, superoxide
anions, H2O2, hydroxl radicals, and
NO Result cell membrane disruption, lipid
peroxidation Side-effect ROI induce deposition
of lignin, callose, and HPRGs
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Host Selective Toxins
HC toxin (Cochliobolus carbonum) acts in
nucleous, causes hyperacetylation of histones
no antifungal compounds AAL toxin (Alternaria
alternata f. sp. lycopersici) inhibits ceramide
synthase in ER, leading to PCD T toxin
(Cochliobolus heterostrophus) ion flux in
mitochondria (Texas CMS) Victorin inhibitor of
glycine decarboxlyase, leads to PCD
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Toxin targets in the plant cell
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Victorin
Victoria blight of oats, caused by Cochliobolus
victoriae victorin absolute requirement for
pathogenicity susceptibility is tightly
associated to resistance to crown rust of oats
(Puccinia coronata) sensitivity conditioned by
the Vb locus, dominant alleles confer
susceptibility inseparable from Pc2 locus,
which confers resistance to crown rust (same
gene?)
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Victorin induces a form of PCD (apoptosis)
shares many of the symptoms of apoptosis,
e.g. DNA laddering, heterochromatin
condensation, cell shrinkage, protease
activation role of caspases? mitochondrial
dysfunction very similar to the HR induced by P.
coronata!!
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