Construction and analysis

1
Construction and analysis of a genomic DNA
library
2-1
2
Construction of a cDNA library
2-2
3
Differences between a genomic and cDNA library
Genomic Library Promoters Introns Intergenic Non-e
xpressed genes
cDNA Library Expressed genes Transcription start
sites Open reading frames (ORFs) Splice points
(Not in Chapter)
4
Purification of mRNA
2-3
5
Synthesis of cDNA from mRNA
2-4
6
SfiI digestion sites
2-5
7
Is there an insert?
A.f. insert
2-6
8
Life cycle of a bacteriophage
2-7
9
Constructing a genomic library in ??phage
2-8
10
Constructing a genomic library in cosmids
2-9
11
Constructing a genomic library in YACs
2-10