???71? (Enterovirus 71)

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???71? (Enterovirus 71)

• ??
• ?????
• ???71????
• ???71??????
• ?????71???????
• ?????71???????
• ???71????????
• ???71??????

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• ?RNA??? picornaviruses
• ???(??????????????)
• ??RNA? 30 nm????????-7400 nt
• ???
• A??????-?23? (coxsackie viruses)
• B??????-?6?
• ??????-?3?(polioviruses)
• ????-?30?(echoviruses)
• ???68?69?70?71?
• ???72?-A?????

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• ??????????
• A??????-??????
• 16?-????(hand-foot-mouth disease)
• B??????-???????????????
• A21?A24??????
• ????
• 11?20 ???????-????

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????? 7400 nt, RNA
P2
P3
P1
3UTR
5UTR
VP4 VP3 VP2 VP1
2A 2B 2C
3A 3B 3C 3D
?????? (4) Structure protein gene
??????? (7) Non-Structure protein gene
UTR untranslation region ????
??????????????
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• ??? 71????- History of EV71
• 1969 ????-??????????????
• 1972 23??????????????
• ??-??????
• ??-??????
• ?????-???????
• 1975 ????-705 ??
• 77.3 ?????
• 21.1 ??????
• ????? HFMD
• 1978 ???-826??
• 87.7 ?????
• 4?? HFMD
• 1985 ????AFP(??????)??

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• 1997 ????????????
• ??????????????
• ???????
• 1998 ????????????????
• (?????????????????)
• Sarawak-34? ????-4?
• ??-???????? 5-7??9-11?
• 13???????????????
• ?????150?-???? 210????
• ??? EV 71?CA 16??
• 405????78?(??????)
• 1999 ?? 6000?
• ????????????
• ??? EV 71?CA 16??
• 2000??????????????

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• ????
• ?????????EV 71?????
• ????6??-3?
• ??????
• ??????

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• EV 71??????
• ????CA5?9?10?16
• 1970 ?? ????????
• 1999 ?? 7???????????
• 1998 ?? 80???????????
• ?????CA8?10?16
• ????????
• ?????
• ??????????????????
• ??????
• ??????????????

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• ??EV71?????
• ???????- ?
• ????????????
• ??????
• ????  (hand-foot-and-mouth disease)
• ??????????????
• ????????, ???????
• ??????(herpangina)
• ???????????,?????
• ????????????????
• ???????
• ????????(acute hemorrgagic conjunctivitis)

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• 1998??EV 71??????????3???
• ?????????-????5???????
• ???????????????,???,20???????
• ???????????????
• ????????(??????)
• ???80,???????????
• ??????????????
• ???????????
• ???????????????
• ?????????????????
• ???B????

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87?96?????????????????
?? ????? ????? ????? ???
87 405 405 78 19
88 107 35 9 26
89 457 291 41 14
90 633 391 58 15
91 315 162 30 18
92 139 70 8 11.5
93 148 50 5 10.0
94 355 142 15 10.6
95 12 0 0
96 11
97 373
98 26
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• ????????????????? 94.8.25.??
• ??????????????
• ????????????????,??? ?????????????????,??????(myoc
  lonic jerks)??????????????????????????????????????
  
• ????????,???????????????????????????????,?????????
  ???????

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Molecular epidemiology and evolution
????????? ??5 UTR?VP1 gene?VP4 gene Molecular
genetics of virulence ????????
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Prospects for control of EV71 infections
EV 71??????? 1. Public health surveillance
??????? 2. Antiviral agents ?????? WIN
?????????,???? ?????,???????(phase III)
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• Vaccine development ?????
• ???????
• ?????-subunit vaccine VP1- DNA??
• ??????????????
• ????EV 71?????
• ??..???
• ????
• ?????-???????EV 71????
• ????????????
• ?????????? infectious cDNA clone

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• Future research should focus on
• understanding the molecular genetics of EV71
  virulence ??EV71????????
• identification of the receptor(s) for EV71 ??????
• development of antiviral agents to ameliorate the
  severity of neurological disease
  ?????????????????
• vaccine development to control epidemics
  ??????????

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5??????? ????????? ??????..(2008/06/13 1637)
?????
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?????10??? ??????????!(2008/07/08 0056)

• ?????????????300?,????????????????????????????????
  ?????????,??10??????????????,???????,????,???????,
  ?????????????,??????????? ??????,???????????????
  ?????,???????????????????????,??????!
  10???????????????,??????????71???,?????????,????
  ??????10???????,??????,?????????????????
  ??????????????,???????????????,?????????????????
  ??,????????????? ??????,?????????????,??????????
  ?????????5000?????????,??20??,????10??,???????????
  ????????,?????????
• ??????????????,????????????,???????????,??????????
  ??????

?????
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????? 9??????? ??????????(2010/05/18 1956)

• ????????????10?????71???,????9??
  ?????,????,??????,???????????????????,???????????
  ???????????,??????,??????,??????????,???????????,
  ?????????,?????? ????????????????????71?????????
  ?5??????,??????,???71???? 1???????????????,???????
  ???,??30???2??

?????