?????(column chromatography)

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????????

• ?????(column chromatography)
• ?????(adsorption chromatography)
• ?????(partition chromatography)
• ???????(ion-exchange chromatography)
• ???????(size-exclusion chromatography)
• ???????(planar liquid chromatography)
• ?????(thin-layer chromatography)
• ????(paper chromatography)
• ???????(high-performance liquid chromatography)

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?-??????

• ?????????????,?????????,???????????????????,??????
  ??,????????????????
• ???????????????????????????????????????,?????????
  ??
• ????????????????????????????????????

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?-??????

• ???GC??,??????????????,??????????????????,????????
  ?????????????
• ????????????,??????????????????????,?????????????
• ????????,??????-????????

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???????

• ???????????,??????,????????????????,??????????????
  ,???????????????????????
• ???????????????????????????????????????,??????
• ????????????????,??????????

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???????

• ???????????????????????,?????????????????,????????
  ???
• ????????????????,???????????????????
• ???????????,?????????????????(ResinOH(s)-)
• ?????????????????,??????????????(Resin-H(s))

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???????

• ??????????????????,????????????????????????
• ????????????????????,??????????????,?????????,????
  ?????,??????
• ??????????,??????????

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?????

• ??????(PC)??????(TLC)
• ?????????????,?????????????????????,?????????

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????

• ??????????3 cm????????,?????,?????????A?,?????????
  ???????,???????????????,????????????????3
  cm??,???????
• ????????,??????????????,??????,???????,?????

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???(ratio of flow Rf)

• Rf????????????????,?????Rf???
• ??????????????????,???????????

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????

• ?????????????Whatman No.1??, Whatman No.2??????,
  Whatman No.4???????
• ?????????????????,???2-5 mm
• ???????????,???-??-???(521)????-?-?(912)
• ??????????????????????????????

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???????

• ??????????????????????????
• ??????????????????????
• ???????????????
• ????????????????????????,????????????

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?????

• ??????????,??????????????????????,????????????
• TLC???????????????(10)???TLC????,????????????,???
  ??????TLC????????
• ?????????????????????,???????????????

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???????

• High performance liquid chromatography HPLC
• ??????????????????,????????????????,??????????????
  ?,????????,????????
• HPLC????????????????

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HPLC???

• ??????????0.5-2 L???????,???????????????????????
• ??????????1000 psi???0.1-10 mL/min???????????????
  
• ????????????????????????
• ????????????
• ????????????GC????,?????UV?????????????

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