PowerPoint Presentation Templates

1
A new method to measure 10B uptake in lung
adenocarcinoma in hospital BNCT
E. Capelli, P. Chiari
F. Basilico, P. Mauri
A. Zanini
D. Bolognini, E.M. Donegani, A. Mattera, M.
Prest, S. Hasan
G. Giannini, E. Vallazza
P. Cappelletti, M. Frigerio, S. Gelosa, A. F.
Monti
F. Borasio
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Outline

• BNCT
• The INFN PhoNeS project
• An innovative approach to Boron Imaging
• Neutron Autoradiography
• Measurements and results
• Conclusion

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Boron Neutron Capture Therapy
10B (n,a,?) 7Li

binary technique
high LET particles

• BNCT could become useful for
• highly-fatal tumours
• Extended (e.g. liver, lung)
• Radioresistant (melanoma)
• Localized near vital organs (glioblastoma)

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Difficulties for BNCT applications
Low toxicity High specificity Non-persistence
in the bloodstream
High intensity thermal-epithermal neutron
beam (5108 n/cm2 s1) lt 10 keV energy range
Gamma contamination lt 2 10-13 Gy cm2 / n
BPA
BSH
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Neutron Autoradiography

• Nuclear reactor
• Solid state track detector (CR 39)
• Sometimes cryogenic temperature during
• the irradiation
• Optic microscope for identification and count

• Hospital-based neutron source
• Real time detector

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PhoNeS (PhotoNeutronSource)
Neutron flux in the treatment cavity F (1.3
0.1) x 107 n cm-2 s-1
?-contamination reduced of 97
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Real-time Neutron Autoradiography
0.5 cm CERROBEND photonconversion
6 cm PMMA thermalization

• IRON BOX
• Samples
• Detector

Neutron flux F (1.5 0.1) x 105 n cm-2 s-1
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The imaging detector
Silicon Dimensions (9.5 x 9.5) cm2
Hamamatsu Microstrip Silicon Detector 768 strips
Readout pitch 242 µm
Read-out by 3 self-triggering TAA1 ASICs
Counts vs position
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The Data Acquisition System

• UNDEPLETED silicon detector

to detect a-particles from boronated samples down
to 25 ng of 10B

• INTER-BUNCH acquisition

18 MeV electrons Bunches of 5 µs Frequency 100 -
300 Hz
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The samples
SOLUTION HOMOGENEITY
SPECIMEN SUPPORT
to prevent crystallization and self-absorption
of the a-particles by the sample itself

• plastic support (liquid solution)
• evaporation
• glass slide (blood samples)
• coagulation process

KNOWN VOLUME
Drops of 5 µl
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Calibrating with BSH
Integrated Spot Content (AU) vs Concentration
(ppm)
500 MU/min 18 MV photons Varian CLINAC 2100
C/D, SantAnna Hospital (Como)
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The first ever kinetic curves
Blood and urine of a patient administered BSH,
taken at different times with respect to the
administration.
URINE
BLOOD
rescaled at the max value obtained by
Si-autoradiography
Special thanks to Dr.Sauerwein
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Ex-vivo lung perfusion
in collaboration with S.Luigi Hospital in
Orbassano (TO), Italy

• Human lung affected by adenocarcinoma
• therapetic resection
• perfusion of blood and BPA

• Blood samples taken at different times
• to estimate 10B uptake

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Kinetic curves

• Blood samples doped with BPA

• Comparison between real-time
  autoradiography to MS analysis

• The absorption takes place only in the first
  90mins

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CR 39
10B concentration in tumour and healthy tissues
of resected lobe
High-resolution images to reproduce the features
of the tissues
Unhealthy tissue
CT / CH 1.5 - 2
Healthy tissue
Special thanks to Dr. Giannini
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Conclusions

• Neutron Autoradiography in a hospital environment
  
  successfully performed - Linac
• Development of an hospital-base neutron source
  
  to perform Boron Imaging with a 1D spatial
  resolution
• Real-time detector to perform pharmacokinetic
  studies
  results during the irradiation
  
• Blood samples from a perfused lung affected by
  adenocarcinoma kinetic curves - 10BPA down to
  concentrations of 5ppm
• Evaluation of 10B concentration in tumour and
  healthy tissues
  Si-autoradiography CR 39
• Looking forward to make BNCT possible in every
  radiotherapic unit

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A new method to measure 10B uptake in lung
adenocarcinoma in hospital BNCT
E. Capelli, P. Chiari
A. Zanini
F. Basilico, P. Mauri
Thank you!
G. Giannini, E. Vallazza
F. Borasio
D. Bolognini, E.M. Donegani, A. Mattera, M.
Prest, S. Hasan
P. Cappelletti, M. Frigerio, S. Gelosa, A. F.
Monti