Last Class Protein-DNA Binding

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Last ClassProtein-DNA Binding

• Helix-turn-Helix, homeodomain, leucine zipper,
  helix-loop-helix, zinc-finger motif
• Homodimer and heterodimer
• Techniques to identify gene sequences bound to a
  known protein or proteins bound to known sequences

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Gene Expression RegulationTranscription
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Tryptophan Gene Regulation
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Tryptophan Gene Regulation
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(No Transcript)
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(No Transcript)
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The binding site of Lambda Repressor determines
its function
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Combinatory Regulation of Lac Operon
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Protein Binding can be enhanced by DNA spacer
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Gene Activation at a distance
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Regulation of an eucaryotic gene
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Functional Domain of gene activation protein
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Gene Activation by the recruitment of RNA
polymerase II holoenzyme
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Gene engineering revealed the function of gene
activation protein
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Chromatin modulation
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Two mechanisms of histone acetylation in gene
regulation
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Synergistic Regulation
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Transcription Events
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5 ways of gene repressor protein to be functional
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(No Transcript)
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Protein Assembly to Regulate Gene Expression
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Integration for Gene Regulation
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Regulation of Gene Activation Proteins
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Insulator Elements
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Summary

• Gene activation or repression proteins
• DNA as a spacer and distant regulation
• Chromatin modulation, TF assembly, polymerase
  recruitment
• combinatory regulations

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Posttranscriptional Regulations
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Posttranscriptional regulation possibilities
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Alternative RNA Splicing
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Alternative Splicing of RNA of the Drosophila
DSCAM gene
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Negative and Positive Controls of Alternative
Splicing
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Antibodies (membrane-bound, secreted) in B
lymphocyte
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RNA Editing
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Nuclear Export
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Localization in cytoplasm
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3 UTR in regulating LocalizationRed intact 3
UTR,Green 3 UTR deleted
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Phosphorylation Regulation
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Internal Ribosome Entry Site (IRES)
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Two Mechanisms of mRNA decay
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The competition between mRNA translation and decay
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Stop Codon CheckingShould after all the exons
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SiRNA Mechanism
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Summary

• Premature termination
• Alternative RNA Splicing
• 3 cleavage and Poly A addition
• RNA editing
• Necleus transportation
• Localization of RNA at the cytoplasm
• Translational initiation
• Degradation