Surface Plasmon Resonance Biosensors

1
??????????
Surface Plasmon Resonance Biosensors
2
Surface Plasmon Resonance
--- SPR is a real-time, label-free, optical
detection method for studying the
interaction of soluble analyte with
immobilized ligand --- completely
non-specific 1902 Wood anomalous diffraction
due to excitation of surface plasma waves 1968
Otto the method of attenuated total
reflection 1988 (1990) BIAcore
3
Total Internal Reflectance
n1 gt n2 evanescent distance 100 nm
4
Thin Conducting Film
A medium of high refractive index A thin layer
with good electric conductivity A medium of low
refractive index
5
Characteristics of SPW
Gold is the most practical. (In) too expansive,
(Na) violently reactive, (Cu, Al) too broad in
their SPR response (Ag) too susceptible to
oxidation
6
Configuration of SPR
7
BIO-SUPLAR 2
8
BIAcore
9
Real-Time Kinetics
10
Sensorgram