Coliforms

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Coliforms
http//www.semp.us/publications/biot_reader.php?Bi
otID109
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Coliform Bacteria

• Bacillus
• Gram-negative
• Non-spore forming
• Ferment lactose
• Citrobacter
• Enterobacter
• Klebsiella
• Escherichia

• Bacterial indicators

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Escherichia coli
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Good Strains

• Vitamin K

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Bad Strains
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Bad Strains

• Haemolytic uremic syndrome (HUS)

• Shiga toxin-producing
• E. coli (STEC)
• O157H7
• O111H8
• O26H11
• O103H2

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Bacteriological Water Analysis

• NC Division of Water Quality (NCDWQ)
• Surface Water Standards
• REDBOOK
• MFTTC
• 200/100 mL
• 14/100 mL

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Water Quality Index

• Fecal coliform measurements
• Membrane Filtration
• Measures both Total Coliform and E. coli
• Multiple-Tube Fermentation
• Most Probable Number (MPN)

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Membrane Filtration using mColiblue