1. ??????????????? (Serial dilution)

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????????????? 2
1. ??????????????? (Serial dilution)
2. ????????????????????????????????????
2
??????????????? (Serial dilution)
0.5 ml
0.5 ml
0.5 ml
0.5 ml
0.5 ml
0.5 ml
1 2 3 4 5
6
????????0.5 ml
Dilution 12 14 18
116 132 control
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???????? Dilution
N1V1 N2V2
N1 dilution ?????????????? V1
????????????????????? N2 dilution
???????????????????? V2 ????????????????????????
????? (V2 V1 ???????????????????????????)
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V1
0.5 ml
V2 0.50.5 1
???????? 0.5 ml
14 ?
N1
N2
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??????????? 1. Tube precipitation test
??????? - ?????????????????????????????????????
???????????? ??????????? Ab
titer ??????????????????
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Serum 0.5 ml
0.5 ml
0.5 ml
0.5 ml
0.5 ml
0.5 ml
1 2 3 4 5
6
Diluent0.5 ml
Dilution 12 14 18
116 132 control
Antigen 1 1
1 1 1 1
???
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Dilution 12 14 18
116 132 control
????? (precipitate)
Ab Titer 18 or 8
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??????????? 2 Immunodiffusion
-???????????????? Ag ??? Ab ???????????? ?
??????????? -?? precipitin band ???????????
(???????????????????)
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??????????? 3. Blood grouping
- ??? ?.?. ?????????????????????????????
??????????????????????? - ??? sterile technique
?????????????????? - ????????-?????????, ???
lancet ????????, ?????????? slide 2 ??? -
??????????????? ? ?????????????????????????????
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???? Agglutination
??????? Agglutination
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????????
1. Single radial immunodiffusion (SRID)
2. Immunoelectrophoresis (IEP)
3. Immunodiffusion (ID)
4. Tube precipitation
5. Enzyme-linked immunosorbent assay (ELISA)
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Enzyme-linked immunosorbent assay (ELISA)
By... Assistance Professor Suwin Wongwajana,MD.
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Microtiter Plate (ELISA Plate)
96 wells plate (8 rows, 12 columns)
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Indirect ELISA (For detection of antibody)
1. Coat plate with Antigen
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2. Wash
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3. Add specimens (with Ab, or No Ab)
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4. Wash
No specific Ab
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4. Add conjugate (anti-human IgGEnzyme)
Y
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5. Wash
(Y)
with specific Ab
No specific Ab
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Y
Ag
Human-IgG
immunized
Y
Y
Y
Rabbit anti-human IgG
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6. Add substrate (Chromogenic)
E
E
Y
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?????? Absorbance (O.D.)
?????????????????????????????????specific
antibody ?????????????
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Immunofluorescence(IF)
Cell
Tissue
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Direct Immunofluorescence (For detection of
antigen)
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Anti-CD4 Fluorescein dye

F
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Fluorescent Microscope
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Indirect Immunofluorescence (For detection of
antigen)
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CA Ag
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??????? ?.?. ??????
1. ????? serial dilution
2. ??????????????????????????????????????
3. ??????????? slide immunodiffusion
4. ?????????????????????????????????????????
????????????
5. Conference ??????????? 22 ??.?.50 8.00-9.00
?. ?? Quiz