Analysis of Coenzyme Q 10

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Analysis of Coenzyme Q 10
School of Pharmacy
School of Pharmacy

• By Taylor Sineath
• 6-1-01

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Purpose

• To test the qualities of Coenzyme Q10 dietary
  supplements using analytical methods.

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What is Coenzyme Q 10?

• Naturally occurring compound found in organisms.
  Used for a variety of indications notably
  cardiovascular diseases.

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Forms/Dosage

• GNC/FoodLion both 10 mg capsules
• GNC is in a capsule form
• FoodLion is in soft gel form

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Testing Methods

• Weight variation
• Disintegration
• HPLC Assay

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Weight variation

• Tests the variation in weight of capsules
• Tests the deviation from the average
• Tests the net fill of soft gel (FoodLion)
• Tests the weight of the capsules (GNC)

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Disintegration

• Tests the solubility in an environment supposed
  to imitate that of the stomach
• Used 6 capsules in sodium acetate buffer
• 37oC /- 2oC
• The limit was 45min

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HPLC Assay

• Determine concentration of analyte within a
  sample
• Measures it by light absorption
• Analyzed 3 capsules per lot

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HPLC Setup

• Used a wavelength of 275nm
• Flow Rate 1ml/min
• Column 150mm x 4.6mm Altima C18 3?
• Mobile Phase (2575) HexanesMethanol
• Injection volume of 20 ul

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Sample Chromatogram
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Sample Preparation Soft gels

• Cut open the shell using forceps and a scalpel
  over a funnel in a 100ml volumetric flask
• Wash the funnel,forceps and the scalpel into the
  100ml flask with 20ml of hexanes
• Sonicate for 5 min and diluted to volume with
  methanol
• Then sonicate again for 5 min

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Sample Prep. (cont.)

• Pipetted 2ml of the sample into a 50ml
  volumetric flask and diluted with methanol
• Filled a HPLC vial 1/2 to 3/4 full

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Data from FoodLion Weight Variation
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Data From GNC Weight Variation
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Disintegration of FoodLion Brand
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Disintegration of GNC Brand
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Assay of FoodLion Soft gels
Lot 9DN0572 8HL701 96N0749
found 106.2 104.7 85.0
/- 24.58 6.3 14.5
mg/cap 10.6 10.5 8.5
mg/cap/- 2.5 0.6 1.5
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Assay of GNC capsules
Lot 82945 82040 88306
found 122.3 52.0 107.2
/- 10.5 58.9 4.3
mg/cap 12.2 5.2 10.7
mg/cap/- 1.1 5.9 0.4
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Conclusions

• Weight variations within brands consistent
• Disintegrations within brands consistent
• Assay would indicate variability within the lot
  and from lot to lot
• Preliminary data suggests that FoodLion would be
  a better choice
• Costs less 10 vs. 17
• 100 capsules vs. 90 capsule in bottle
• Less variability