Chromatography

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Lecture 22

• Chromatography II
• HPLC

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Mobile phase
Stationary phase
Time for solvent molecule is the same as before
tm
Time for analyte molecules is different tr(1)
and tr(2)
tm
tr(1)
Selectivity factor Separation factor Relative
retention
tr
tr(2)
time
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Distribution coefficient
Retention factor
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A 0
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B
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Nonpolar phase
Polar phase
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