Instrumental Analysis ? ?

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Instrumental Analysis? ?
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2

• ????????????????
• (1)???????????,??????????????
• (2)????????,?????????????,???????????

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• ???????(???????)
• (1)????,?????,??????
• (2)????
• (3)????,?????,???????
• (4)??????(5)
• (5)??????????????

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????????

• ??-?????? Ultra-Visable Spectrophotometry
  (UV-SP)
• ??????? Fluorescence spectrometry
• ???????? Atomic Absorption Spectrophotometry(AAS
  )
• ??? Electrochemistry
• ?? Chromatography
• High performance liquid
  chromatography(HPLC)
• Gas chromatography(GC)

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??-???????
???? 1.??????????? 2.???????? 3.??????? 4.?????
?? 5.????
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??-???????
???????????????????(200760nm)?????,??????????????
?????????
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??????

• ??????????????????,??????,???????????????????
  ?????
• ???????????????????????

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????
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• ????????
• ?????????
• ?????????????????
• ????????????????

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????
?????????????????
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?????????

• 1.????????????
• E??EeEvEr
• Eegt Evgt Er?

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?E??gt?E??gt?E??
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• ????
• ????????????????????????????????????
  ??
• ????????????,???????????????,???????????
  ????????(?EE1-E2hc/?)????????????????? M(??)
  h? ? M(???)

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• ??????????, ?????????????????,??????????
  ???,???????????????????????????,??????????

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• ?????????

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• ??????????????????(A),?????????,??????????,
  ????????????(????)???????????????????

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•   ????
• (1)???
• (2)???????????????????,???????,??????????????
  ??????????(A)????????,A??????,?????????????????
• (3)????????

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(No Transcript)
19
??????????

• a.???????????????,???????????
• b.????????,????????lmax???

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????????????????
21

•   ??
• (1)?????????
• (2)??
• (3)??????

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???????

• ??????????????????
• (1) ????????????????????????????????????????
  ?????????????????????,???????????????,?????

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• ??????????????????????????,??????????????????
  ??????,???????,????

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• ?????????????????????????
• ????????????????
• ???????????????
• ???????

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• ??????????,??????????????????????????????
  ??,?7?????????????????
• ?????????????????,???????????

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??????
27
???????

• ???????????????
• ??????????????,?????????????????
• ????????????,???????????,??????,?????????
  ????????????,?????????????????????????????,???????
  ?

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??????????
???
????
??????
????
????
?????
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• ????????????????,??????.

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? 1,10??????????????
?????????? ????????,???510nm?,????600nm?
????????,??????????????,????????,?1,10????????????
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• ???????
• ????(10200nm)
• ????(200400nm)
• ????(400760nm)
• ????O2?CO2?H2O???10200nm????,??????,?
  ??????(???????????),?????????????????????

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?????????? (??)
????????p?p, n?p

• ????
• (1)?????????????????????????,???????????
• (2)?????????????????????????,???????????

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• (3)???????????????????????????????
• (4)???????????????????????????????

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• (5)????????????????pp?n-p??,?????????????????
• (6)????????????????????????????????(???)?

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??????

• ??????-??????????
• ???????????????????????,?????????????????????
  ???

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Alg(I0/It)KbC

• ?C???,A ? bLamberts law
• ?b???,A ? CBeers law

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• ????
• TIt/ I0,?????(transmittance) ,???????????????
• ????LgIo/It1/T0.434 k,bckbc
• ALg1/TLgIO/Itkbc,A-????(absorbance)
  ,????????????
• ?????? optical density,OD
• ??? extinction,E

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??????K

• 1?a(absorptivity)-???????1g/L????????1cm?????,???
  ???????????
• 2?e(molar absorptivity)-?????????1mol/L????????1c
  m?????,???????????????

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• 3?E11cm-???????????????1(W/V)????????1cm?????,?
  ??????????????
• ?????????eaM E11cm.M/10,??????e?

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• 4?????????????????A?,?????????????,????????????
  
• 5???????????????????????????????????????????????
  ??

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• ???????????????0.002???,??1cm????,?278nm???
  ??????A?0.614,??????????????????E11cm?(????323.15
  )

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• ????e?????
• (1)e??????????e???????????,???????????,?????????
  ?????????????????????????,?????,?????????e????

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• (2)e????????????????????????,????????

• ??e??10105??,egt104????,e?103104?????,elt103??
  ???

?????,e120????(DDTC)??,e12800,??????,e158
000?
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?????
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722?????
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• ????????????

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(No Transcript)
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• ????
• 1???????
• 2????????????????
• 3???????????????????

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• ????????????????
• (1)?????320nm2500nm????????????????????,???????
  ??

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• (2)??????????????,???????????????,?????

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• ???????180375nm?????,???????????,????????,????
  ????????

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????!