??????????????(ES?????????)??,?????????????????,???????????????????

1
?????????????

• ?????????

2
??????????
3
???

• ?????????????????,????????????????????,???????????
  ???,????????????????????

4
?????????????????

• ???????
• ???????????????????????
• ????????,??????????????????????
• ????,???????????????????

5
??????????????(ES?????????)??,???????????????
??,???????????????????

• ???????,??????????????????????????????
• ??,???????????????,??????????????,?????????????,??
  ?????????????

6
???????,????????????????,????????????????????
7
?????????

• 2006?11?20?,???????????(Shinya Yamanaka)??????????
  ??????(James Thomson)??????????????????,?????
  ???????,????????????????,??????????????????

8
??????????????11?20???????
9
????11?20????????????
10
2006?8?,??????????????????????
11
????????????

• 2006?,Takahashi ? Yamnaka ??????????????????????,?
  ???????????????????,???????????????(induced
  pluripotent stem cells,iPS??)?????????????????????
  ????????????????????????,???????????????????

12
?????????????????????,??????????????????????

• ?????????
• ?????????
• ???????????????????

13
?????????

• 1.??????????????????????,????????????????????????
  ????????????DNA?????????????????????????X???????

14

• 1.1.DNA???
• 1.2.??????????,?Oct-4
• 2.?????
• ????????A?B?C??????????

15
(No Transcript)
16
3.??????miRNA

• miRNA?????????????
• ???miRNA?????????????????????

17

• ???,?????????????????????????????????????????????
  ????????????????,???????????,???????????4???
  (Oct4,Sox2,c-myc?Klf4 ),????????,?????????????????
  ??????????iPS???

18

• ????????????????????????Oct4,Sox2,c-myc?Klf4??????
  ?????

19
1.Oct3/4

• Oct-4(??Oct-3)??POU?????????13 ?
• ???????????????????????????,?????ICM??????????????
  ?????????

20
2.SOX2??

• SOX2??????????????(master genes)???????????????DNA
  ???????????????

21
3.????c-MYC

• ????c-MYC,??????????????????,????????????????????,
  ?????ES??????

22
4.Klf4

• Krueppel-like factor 4,KLF4????????Klf4
  (??GKLF)????????????

23
iPS??????
?????? Oct3/4?Sox2?c-Myc?Klf4
??????
?????
Ips??
????????????
24
???????
25
(No Transcript)
26

• ??????????????????????????????,????????????Ips????
  ????Ips?????????????

27
Ips???????(????)

• ?????????????????????????????????????????????????
  ??????????,????????????????

• ???????????? ES??-??????????????????????????????
  ES?????????????????????,ES??????????,?????????????
  ??????

28
(No Transcript)
29
Ips???????(??????)

• Ips????????Takahashi?Yamanaka?2006???????????????
  ?????????????????4???????????Oct4,Sox2,c-myc?Klf4
  ??????????Fbx15???????????????,?????ips??,????????
  ?????????????

• ??????????????????????,??????????????????????????
  ?????????????????c-Myc,?ips?????????

30
Ips????????????(?????????)

• ???????ES????????????,???????????????????????24???
  ?????????????????????,??????????,??????10???????4?
  ???????,?????????????????,?4???????2??3??????????E
  S?????ips????????Oct4,Sox2,c-myc?Klf4?4???

31

• ??ES????????????????????????????????ES????????????
  ??,??????ES??????????????,??????ES????????????????
  ??????????????????????,????14?????????????????????
  ?????ES??????????????,???????????????????????????4
  ???Oct4,Sox2,Nanog,Lin28.

32
Ips????????????(Ips?????)

• Ips????????????????????????????????????????
  ???????Fbx15?ips??????,???Fbx25???????????????????
  ?????,??Fbx25????ips????????
• ????????????????????Maherali????,?????????????
  ???ips????????????????????????????????,???????????
  ????????????????ips???
• ???????????????????????,???????????Nanog?Oct4????
  ??????????????????,?????????????????ips???,???????
  ????????????????

33
iPS????????????
34

• 1???iPS????????,??????????,????????????????
• 2????????????,????????,????????
• 3????????????????????????
• 4????????????iPS??,??????????????

35
??????????????????

• ????Whitehead???????Wernig?????,??Oct4?Sox2?Klf4?c
  -Myc??????????iPS??????????,???????????????

36
????????iPS??????????,??iPS???????????????????,??
??????????????

????iPS??
iPS????????????
37

• ??,??????????iPS??,???????????????9??(?????),?????
  ,iPS???????????????,??????????????????,???????????
  ?????,??????????????????????????????,?????????????
  ??????,???????????

iPS???????????????
iPS??????????????
iPS??????????????????
38

• ?????????????,iPS??????????????????,???????????,??
  ????????,????????????????????????

iPS???????????????????
39

• ??????????????????6-?????,????????????????????????
  ???????,?????????????,????????????????????????????
  ???,??????????????

40

• ??,????iPS????????,????iPS???????????,????????????
  ?????(??6-??????????)?????????,10?????????9???????
  ?????,?????1??????????????????

41
Ips??????????

• 1???????,?c-myc???,????????
• 2?????????????????????,????????????????
• 3????????ESC????????
• 4?????,?????0.1?

42
??
43
(No Transcript)
44
???????

• ???????????(???),??(???),??(??)
• ??????????????????????????????(???),??(???),??(??
  ?)
• Ips????????Ips????????????????(??),??(???),??(???
  )
• Ips????????????????(???)

45
????