A 3-week Biotech Unit for Any Biology Class

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A 3-week Biotech Unitfor Any Biology Class
Ellyn Daugherty SMBCP, San Mateo
CA Educator/Author www.BiotechEd.com www.SMBiotech
.com www.emcp.com/biotech www.sargentwelch.com/bio
tech
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Biotech in Biology3 weeks (15 hours)
gt What is Biotechnology? Computer Search
Collage gt Micropipeting Activity gt DNA Spooling
Activity gt DNA Fingerprinting Using RFLPs
Activity gt Recombinant DNA Technology gt Bacterial
Transformation (GMO) Activity gt Bioethics of
Engineering Organisms gt Careers in Biotechnology
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Materials, Supplies, Funds Needed for Biotech in
Biology Unit

• Class set of
• 4 micropipets/tips each (P-1000, P-200, P-20)
• 4 gel boxes, 2 power supplies
• DNA Spooling Activity Kit
• DNA Fingerprinting Activity Kit
• Bacteria Transformation Activity Kit
• Other supplies and materials (96-well plates,
  food coloring, 37C incubation oven, water bath

about 300 in kits/ class about 3000 for
school set of micropipets and gel apparatus
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What Is Biotechnology?

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Micropipeting Activity
Show me Pipet 135 µL or 235 µL into a tube
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DNA Spooling Activity
What Does DNA Look Like? (Edvotek S-10) one
50 min class period (30 min activity, 20 min
discussion 39 / 10 lab groups
Do Onions Have DNA (or other fruits or veggies) ?
(Edvotek S-75) one 50 min class period (30
min activity, 20 min discussion 49 / 5 lab groups
or use my Lab 4b Materials from SW List
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DNA Fingerprinting Using RFLPs Activity
Wards DNA Detectives Lab Activity 109) four
50 min class periods 115 / 8 lab groups

Edvoteks DNA Fingerprinting by Restriction
Fragmentation Patterns 109) four 50 min class
periods 79 / 6 lab groups
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Recombinant DNA Technologyuse http//www.dnalc.or
g/resources/animations/

• Use the Restriction Digestion animation to
  discuss
• Restriction enzymes
• DNA ligase
• Recombinant DNA (rDNA)

• Use the Transformation animation to discuss
• Insertion of rDNA vector
• Selection and Expression of newly insert genes
• Applications of rDNA technology

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Bacterial Transformation (GMO) Activity
Wards Improved Bacterial Transformation Using
GFP 36 V 6359 four 50 min class periods 85/
8 lab groups

Edvoteks Transformation with Green Fluorescent
Protein 223 and 969 four 50 min class
periods 110/ 10 lab groups
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Biotechnology Career Research e-Project

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Bioethics of Engineered Organisms

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Materials Lists www.sargentwelch.com/biotech
Find your Sales Rep for materials/equipment/
support
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Biotechnology Science for the New Millennium
Ellyn Daugherty ellyn_at_BiotechEd.com

• Text with Encore CD
• Lab Manual
• Instructors Guide
• Course Planner (Lesson Plans)
• Instructor Resource Center on web
• Student Notebook, Test Generator, etc. available

www.BiotechEd.com for tour through the entire
curriculum, ancillaries, websites, and
professional development opportunities