Experiment 1 medical microbiology experiment

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Experiment 1 medical microbiology experiment

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Microbic Morphology
Medical microbiology experiment (1)
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• Content
• 1. Microbic Morphology
• ?Bacteria
• ?Virus
• ?Fungus
• 2. Use oil-immersion lens of microscope
• 3.Bacterial gram stain

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• 1. Microbic Morphology
• ?Bacteria
• Demonstration
• ground form
• coccus(Staphylococcus aureus)
• bacillus(Escherichia coli)
• spirobacteria
• vibrio(Vibrio cholerae)
• special structure
• flagellum(Salmonella typhi)
• capsule(Streptococcus pneumoniae)
• spore(Clostridium tetani)
• pilus

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• Coccus

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• Staphylococcus Streptococcus Diplococcus

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• Bacillus

Bacillus anthracis
Escherichia coli
Brucella
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Corynebacterium
Bifidobacterium
Mycobacterium
Clostridium
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• Spirobacteria

Vibrio Spirillum
Helicobacterium
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• Special structure
• Flagellum

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• Capsule
• Spore

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• ? Virus

poxvirus poliovirus
rhinovirus
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tobacco mosaic virus
phage
rabies virus
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• ? Fungus

Unicellular fungusyeast,yeast like
fungus,pseudohypha multicellular
fungusfilamentous fungus,mold,hypha and spore?
S. albicans
Aspergillus
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• 2. Use oil-immersion lens of microscope

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• 3.Bacterial gram stain
• (Staphylococcus aureus , Escherichia coli)
• Gram Stain - developed Christian Gram in 1884
• The gram stain is the most widely used staining
  procedure in bacteriology. It is called a
  differential stain since it differentiates
  between gram-positive and gram-negative bacteria.
  Bacteria which stain purple with the gram
  staining procedure are termed gram-positive
  those which stain pink are said to be
  gram-negative.
• Gram-positive and gram-negative bacteria stain
  differently because of fundamental differences in
  the structure of their cell walls.

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• Principle

Structure of a Gram-Negative Cell Wall
Structure of a Gram-Positive Cell Wall
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• Aseptic technique

Aseptic area 10-20cm
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procedure

• Smear preparation
• Smear
• Dry Fix

aseptic technique
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• Stain procedure
• Initial stain Mordanting
  Decolorize After stain
• (crystal violet 1) ( iodine solution 1)
  (95 alcohol 30) (diluted carbol
• 
  
  fuchsin 30)
• Rinse Blot Microscopic
  examination

rinse
rinse
rinse
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Notice

• 1. Aseptic technique
• 2. Not too much NS and bacteria
• 3. Time of decolorization

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• Material
• 1.????????????????1?/??(4?)
• 2.??1?/??
• 3.??1?/?

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• ?Content of report
• 1.Bacterial gram stain (Principle, Procedure,
  result)
• 2.Bacterial morphology (result examined under
  microscope)

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name magnification result